Microscopic and spectroscopic characterization of rice and corn starch

Starch granules from rice and corn were isolated, and their molecular mechanism on interaction with alpha-amylase was characterized through biochemical test, microscopic imaging, and spectroscopic measurements. The micro-scale structure of starch granules were observed under an optical microscope and their average size was in the range 1-100 mu m. The surface topological structures of starch with micro-holes due to the effect of alpha- amylase were also visualized under scanning electron microscope. The crystallinity was confirmed by X-ray diffraction patterns as well as second-harmonic generation microscopy. The change in chemical bonds before and after hydrolysis of the starch granules by alpha- amylase was determined by Fourier transform infrared spectroscopy. Combination of microscopy and spectroscopy techniques relates structural and chemical features that explain starch enzymatic hydrolysis which will provide a valid basis for future studies in food science and insights into the energy transformation dynamics.

Pallen, Sparsha (författare)
Umashankar, Suchitta (författare)
Mal, Sib Sankar (författare)
Kaniyala Melanthota, Sindhoora (författare)
Mahato, Dhani RamUmeå universitet,Kemiska institutionen(Swepub:umu)dhra0002 (författare)
Zhuo, Guan-Yu (författare)
Mahato, Krishna Kishore (författare)
Mazumder, Nirmal (författare)
Umeå universitetKemiska institutionen (creator_code:org_t)

Ingår i:Microscopy research and technique (Print): John Wiley & Sons83:5, s. 490-4981059-910X1097-0029

Av författaren/redakt...: Govindaraju, Ind ...; Pallen, Sparsha; Umashankar, Such ...; Mal, Sib Sankar; Kaniyala Melanth ...; Mahato, Dhani Ra ...; visa fler...; Zhuo, Guan-Yu; Mahato, Krishna ...; Mazumder, Nirmal; visa färre...

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