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  • Dwibedi, Chinmay KumarUmeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Klinisk bakteriologi,Swedish Defense Research Agency, Umeå, Sweden (author)

Biological amplification of low frequency mutations unravels laboratory culture history of the bio-threat agent Francisella tularensis

  • Article/chapterEnglish2020

Publisher, publication year, extent ...

  • Elsevier,2020
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:umu-168168
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-168168URI
  • https://doi.org/10.1016/j.fsigen.2019.102230DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Errata: Dwibedi C, Larsson P, Ahlinder J, Lindgren P, Myrtennäs K, Granberg M, et al., Corrigendum to "Biological amplification of low frequency mutations unravels laboratory culture history of the bio-threat agent Francisella tularensis". Forensic Sci. Int.: Genet. 45 (2020) 102230. DOI: 10.1016/j.fsigen.2024.103063.
  • Challenges of investigating a suspected bio attack include establishing if microorganisms have been cultured to produce attack material and to identify their source. Addressing both issues, we have investigated genetic variations that emerge during laboratory culturing of the bacterial pathogen Francisella tularensis. Key aims were to identify genetic variations that are characteristic of laboratory culturing and explore the possibility of using biological amplification to identify genetic variation present at exceedingly low frequencies in a source sample. We used parallel serial passage experiments and high-throughput sequencing of F. tularensis to explore the genetic variation. We found that during early laboratory culture passages of F. tularensis, gene duplications emerged in the pathogen genome followed by single-nucleotide polymorphisms in genes for bacterial capsule synthesis. Based on a biological enrichment scheme and the use of high-throughput sequencing, we identified genetic variation that likely pre-existed in a source sample. The results support that capsule synthesis gene mutations are common during laboratory culture, and that a biological amplification strategy is useful for linking a F. tularensis sample to a specific laboratory variant among many highly similar variants.

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  • Larsson, PärSwedish Defense Research Agency, Umeå, Sweden (author)
  • Ahlinder, JonSwedish Defense Research Agency, Umeå, Sweden (author)
  • Lindgren, PetterSwedish Defense Research Agency, Umeå, Sweden (author)
  • Myrtennäs, KerstinSwedish Defense Research Agency, Umeå, Sweden (author)
  • Granberg, MalinSwedish Defense Research Agency, Umeå, Sweden (author)
  • Larsson, EvaSwedish Defense Research Agency, Umeå, Sweden (author)
  • Öhrman, CarolineSwedish Defense Research Agency, Umeå, Sweden (author)
  • Sjödin, AndreasSwedish Defense Research Agency, Umeå, Sweden (author)
  • Stenberg, Per,1974-Umeå universitet,Institutionen för ekologi, miljö och geovetenskap,Swedish Defense Research Agency, Umeå, Sweden(Swepub:umu)perstg96 (author)
  • Forsman, MatsSwedish Defense Research Agency, Umeå, Sweden (author)
  • Johansson, Anders,1966-Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Klinisk bakteriologi(Swepub:umu)anjo0135 (author)
  • Umeå universitetMolekylär Infektionsmedicin, Sverige (MIMS) (creator_code:org_t)

Related titles

  • In:Forensic Science International: Elsevier451872-49731878-0326

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