Chemical Composition, Antimicrobial activity, in Vitro Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans

Sökning: L773:2076 0817 > Chemical Compositio...

In this study, the essential oil of Origanum vulgare was evaluated for putative antibacterial activity against six clinical strains and five reference strains of Aggregatibacter actinomycetemcomitans, in comparison with some antimicrobials. The chemical composition of the essential oil was analyzed, using chromatography (CG) and gas chromatography-mass spectrometry coupled (CG-MS). The major compounds in the oil were Carvacrol (32.36%), α-terpineol (16.70%), p-cymene (16.24%), and Thymol (12.05%). The antimicrobial activity was determined by an agar well diffusion test. A broth microdilution method was used to study the minimal inhibitory concentration (MIC). The minimal bactericidal concentration (MBC) was also determined. The cytotoxicity of the essential oil (IC50) was <125 µg/mL for THP-1 cells, which was high in comparison with different MIC values for the A. actinomycetemcomitans strains. O. vulgare essential oil did not interfere with the neutralizing capacity of Psidium guajava against the A. actinomycetemcomitans leukotoxin. In addition, it was shown that the O. vulgare EO had an antibacterial effect against A. actinomycetemcomitans on a similar level as some tested antimicrobials. In view of these findings, we suggest that O.vulgare EO may be used as an adjuvant for prevention and treatment of periodontal diseases associated to A. actinomycetemcomitans. In addition, it can be used together with the previously tested leukotoxin neutralizing Psidium guajava.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Odontologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dentistry hsv//eng
Aggregatibacter actinomycetemcomitans
periodontitis
Origanum vulgare
essential oil
antimicrobial activity
minimum inhibitory concentration
minimal bactericidal concentration
cytotoxicity
leukotoxin neutralization
biokemisk farmakologi
biochemical pharmacology

Johansson, Anders,1955-Umeå universitet,Institutionen för odontologi,Aa-gruppen(Swepub:umu)anjo0002 (författare)
Yagoubi, Maâmar (författare)
Haubek, DorteAarhus University Denmark (författare)
El Hamidi, AdnaneMohammed V University in Rabat, Morocco (författare)
Rida, SanaMohammed V University in Rabat, Morocco (författare)
Claesson, RolfUmeå universitet,Institutionen för odontologi(Swepub:umu)rolf0001 (författare)
Ennibi, OumKeltoumMohammed V University in Rabat, Morocco (författare)
Umeå universitetInstitutionen för odontologi (creator_code:org_t)

Av författaren/redakt...: Akkaoui, Sanae; Johansson, Ander ...; Yagoubi, Maâmar; Haubek, Dorte; El Hamidi, Adnan ...; Rida, Sana; visa fler...; Claesson, Rolf; Ennibi, OumKelto ...; visa färre...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Mikrobiologi ino ...

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Odontologi

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