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- Stöven, SvenjaUmeå universitet,Umeå centrum för molekylär patogenes (UCMP) (Medicinska fakulteten),Klinisk bakteriologi,Dan Hultmark (author)
Caspase-mediated processing of the Drosophila NF-kappaB factor Relish.
- Article/chapterEnglish2003
Publisher, publication year, extent ...
- 2003
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:umu-17059
- https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-17059URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- The NF-kappaB-like transcription factor Relish plays a central role in the innate immune response of Drosophila. Unlike other NF-kappaB proteins, Relish is activated by endoproteolytic cleavage to generate a DNA-binding Rel homology domain and a stable IkappaB-like fragment. This signal-induced endoproteolysis requires the activity of several gene products, including the IkappaB kinase complex and the caspase Dredd. Here we used mutational analysis and protein microsequencing to demonstrate that a caspase target site, located in the linker region between the Rel and the IkappaB-like domain, is the site of signal-dependent cleavage. We also show physical interaction between Relish and Dredd, suggesting that Dredd indeed is the Relish endoprotease. In addition to the caspase target site, the C-terminal 107 aa of Relish are required for endoproteolysis and signal-dependent phosphorylation by the Drosophila IkappaB kinase beta. Finally, an N-terminal serine-rich region in Relish and the PEST domain were found to negatively regulate Relish activation.
Subject headings and genre
- Animals
- Base Sequence
- Caspases/*metabolism
- Cells; Cultured
- Chloramphenicol O-Acetyltransferase/genetics
- DNA Primers
- Drosophila Proteins/*genetics/metabolism
- Drosophila melanogaster/*immunology
- Gene Deletion
- Gene Expression Regulation
- Genes; Reporter
- Kinetics
- Molecular Sequence Data
- Phosphorylation
- Polymerase Chain Reaction
- Sequence Deletion
- Transcription Factors/*genetics/metabolism
- beta-Galactosidase/genetics
Added entries (persons, corporate bodies, meetings, titles ...)
- Silverman, Neal (author)
- Junell, Anna (author)
- Hedengren-Olcott, MarikaUmeå universitet,Umeå centrum för molekylär patogenes (UCMP) (Medicinska fakulteten),Dan Hultmark (author)
- Erturk, Deniz (author)
- Engstrom, Ylva (author)
- Maniatis, Tom (author)
- Hultmark, DanUmeå universitet,Umeå centrum för molekylär patogenes (UCMP) (Medicinska fakulteten),D(Swepub:umu)dahu0001 (author)
- Umeå universitetUmeå centrum för molekylär patogenes (UCMP) (Medicinska fakulteten) (creator_code:org_t)
Related titles
- In:Proc Natl Acad Sci U S A100:10, s. 5991-60027-8424
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