id:"swepub:oai:DiVA.org:umu-172"
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- Gustafson, Inga,1954-Umeå universitet,Kemiska institutionen (författare)
Phospholipid membranes in biosensor applications : Stability, activity and kinetics of reconstituted proteins and glycolipids in supported membranes
- BokEngelska2004
Förlag, utgivningsår, omfång ...
- 2004
- 123 s.
- electronicrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:umu-172
- ISBN:9173055476
- https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-172URI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
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- SwePubSwePub
Klassifikation
Serie
- FOI-R,1650-1942 ;987
Anmärkningar
- In this study the formation of supported membranes onto planar solid supports has been investigated. The stability and activity of reconstituted membrane receptors has been studied. The potential use of such preparations in biosensor applications is discussed.The lipid films were made by the Langmuir Blodgett and by the liposome fusion techniques. These supported films were characterised by ellipsometry, atomic force microscopy, surface plasmon resonance (SPR) and resonant mirror techniques. The thickness of the films was in agreement with that of a cell membrane. The kinetics of formation of the lipid films was studied and discussed.The proteins, bacteriorhodopsin, cytochrome oxidase, acetylcholinesterase and the nicotinic acetylcholine receptor were reconstituted into the supported membrane. The subsequent analysis showed that the proteins were individually distributed and that the activity was retained, in some cases for several weeks after immobilisation.The glycolipids, GM1, GM2, GD1b, asialo-GM1, globotriaosylceramide, lactosylceramide and galactosylceramide, were also reconstituted into the supported membranes. Their specific interaction with the toxin ricin or with its B-chain was examined using SPR. The affinity of intact toxin and of its B-chain differed markedly and was pH dependent. The carbohydrate chain length and charge density of the glycolipids also influenced the affinity.
Ämnesord och genrebeteckningar
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- Biochemistry
- Phospholipid films
- Liposomes
- Membrane proteins
- Glycolipids
- Ellipsometry
- Langmuir Blodgett (LB)
- Surface plasmon resonance (SPR)
- Resonant mirror (RM)
- Atomic force microscopy (AFM)
- Biosensors
- Biokemi
- Biochemistry
- Biokemi
- Biochemistry
- biokemi
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Puu, Gertrud (preses)
- Lindskog, Sven (preses)
- Konradsson, Peter,ProfessorIFM, avdelningen för Kemi, Linköping (opponent)
- Umeå universitetKemiska institutionen (creator_code:org_t)
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