SwePub
Sök i LIBRIS databas

  Extended search

L773:1010 4283
 

Search: L773:1010 4283 > (2020-2021) > Evaluating macropha...

Evaluating macrophage migration inhibitory factor 1 expression as a prognostic biomarker in colon cancer

Ohlsson, Lina (author)
Umeå University,Umeå universitet,Immunologi/immunkemi
Lindmark, Gudrun (author)
Lund University,Lunds universitet,Kirurgi,Forskargrupper vid Lunds universitet,Surgery,Lund University Research Groups
Hammarström, Marie-Louise (author)
Umeå University,Umeå universitet,Immunologi/immunkemi,Marie-Louise Hammarström
show more...
Hammarström, Sten (author)
Umeå University,Umeå universitet,Immunologi/immunkemi,Marie-Louise Hammarström
Sitohy, Basel (author)
Umeå University,Umeå universitet,Onkologi,Immunologi/immunkemi
show less...
 (creator_code:org_t)
2020-06-09
2020
English.
In: Tumor Biology. - : Sage Publications. - 1010-4283 .- 1423-0380. ; 42:6
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • OBJECTIVE: Several studies indicate that macrophage migration inhibitory factor 1 plays a role for tumor progression in colon cancer. We investigated whether determination of migration inhibitory factor 1 mRNA expression levels in lymph nodes of colon cancer patients could be used as a prognostic marker.METHODS: Expression levels of migration inhibitory factor 1 and carcinoembryonic antigen mRNAs were assessed in primary tumors and regional lymph nodes of 123 colon cancer patients (stages I-IV), and in colon cancer- and immune cell lines using quantitative reverse transcriptase-polymerase chain reaction. Expression of migration inhibitory factor 1 protein was investigated by two-color immunohistochemistry and immunomorphometry.RESULTS: Migration inhibitory factor 1 mRNA was expressed at 60 times higher levels in primary colon cancer tumors compared to normal colonic tissue (medians 8.2 and 0.2 mRNA copies/18S rRNA unit; p < .0001). A highly significant difference in mRNA expression levels was found between hematoxylin-eosin positive lymph nodes and hematoxylin-eosin negative lymph nodes (p < .0001). Migration inhibitory factor 1 and carcinoembryonic antigen proteins were simultaneously expressed in many colon cancer-tumor cells. Kaplan-Meier survival model and hazard ratio analysis, using a cutoff level at 2.19 mRNA copies/18S rRNA unit, revealed that patients with lymph nodes expressing high levels of migration inhibitory factor 1 mRNA had a 3.5-fold (p = .04) higher risk for recurrence, associated with a small, but significant, difference in mean survival time (7 months, p = .03) at 12 years of follow-up.CONCLUSION: Although migration inhibitory factor 1 mRNA expression levels were related to severity of disease and lymph node analysis revealed that colon cancer patients with high levels had a shorter survival time after surgery than those with low levels, the difference was small and probably not useful in clinical practice.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

Keyword

Macrophage migration inhibitory factor
carcinoembryonic antigen
colon cancer
cumulative survival curves; recurrence risk
disseminated tumor cells
immunofluorescence
immunohistochemistry
immunomorphometry
quantitative reverse transcriptase–polymerase chain reaction
carcinoembryonic antigen
colon cancer
cumulative survival curves; recurrence risk
disseminated tumor cells
immunofluorescence
immunohistochemistry
immunomorphometry
Macrophage migration inhibitory factor
quantitative reverse transcriptase–polymerase chain reaction

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

To the university's database

Search outside SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view