Crystallization and preliminary studies of the DNA-binding runt domain of AML1.

• The acute myeloid leukaemia 1 (AML1) protein belongs to the Runx family of transcription factors and is crucial for haematopoietic development. The genes encoding Runx1 and its associated factor CBF beta are the most frequent targets for chromosomal rearrangements in acute human leukaemias. In addition, point mutations of Runx1 in acute leukaemias and in the familial platelet disorder FPD/AML cluster within the evolutionary conserved runt domain that binds both DNA and CBF beta. Here, the crystallization of the Runx1 runt domain is reported. Crystals belong to space groups C2 and R32 and diffract to 1.7 and 2.0 A resolution, respectively.

Nyckelord

acute myeloid leukaemia 1 protein

Runx1

runt domain

transcription factors

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)