Focal adhesion kinase is not required for integrin function or viability in Drosophila.

• The mammalian focal adhesion kinase (FAK) family of non-receptor protein-tyrosine kinases has been implicated in controlling a multitude of cellular responses to the engagement of cell-surface integrins and G-protein-coupled receptors. The high level of sequence conservation between the mammalian proteins and the Drosophila homologue of FAK, Fak56, suggested that it would have similar functions. However, we show here that Drosophila Fak56 is not essential for integrin functions in adhesion, migration or signaling in vivo. Furthermore, animals lacking Fak56 are viable and fertile, demonstrating that Fak56 is not essential for other developmental or physiological functions. Despite this, overexpressed Fak56 is a potent inhibitor of integrins binding to the extracellular matrix, suggesting that Fak56 may play a subtle role in the negative regulation of integrin adhesion.

Nyckelord

Animals

Blotting; Southern

Blotting; Western

Cell Membrane/metabolism

Cytoskeleton/metabolism

DNA/metabolism

Down-Regulation

Drosophila

Drosophila Proteins

Drosophila melanogaster

Focal Adhesion Kinase 1

Focal Adhesion Protein-Tyrosine Kinases

Gene Deletion

Gene Expression Regulation

Immunoblotting

Integrins/*metabolism

Models; Genetic

Muscles/embryology

Mutation

Phenotype

Phosphorylation

Protein Structure; Tertiary

Protein-Tyrosine Kinases/*genetics/*physiology

Signal Transduction

Transgenes

Tyrosine/metabolism

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)