Profiling of acylated homoserine lactones of Vibrio anguillarum in vitro and in vivo: Influence of growth conditions and serotype.

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Buchholtz, Christiane (författare)

Profiling of acylated homoserine lactones of Vibrio anguillarum in vitro and in vivo : Influence of growth conditions and serotype.

Artikel/kapitelEngelska2006

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2006
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LIBRIS-ID:oai:DiVA.org:umu-17991
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-17991URI

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Språk:engelska
Sammanfattning på:engelska

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Vibrio anguillarum produces several interlinked acylated homoserine lactone (AHL) signal molecules which may influence expression of its virulence factors such as exoprotease production and biofilm formation. Using both thin layer chromatography and HPLC-high resolution mass spectrometry (HPLC-HRMS), we demonstrate in this study that the same types of AHLs are produced by many serotypes of V. anguillarum and that altering in vitro growth conditions (salinity, temperature and iron concentration) has little influence on the AHL-profile. Most strains produced N-(3-oxodecanoyl)-l-homoserine lactone (3-oxo-C10-HSL) and N-(3-hydroxy-hexanoyl)-l-homoserine lactone (3-hydroxy-C6-HSL) as the dominant molecules. Also, two spots with AHL activity appeared on TLC plates, which could not be identified as AHL structures. Trace amounts of N-(3-hydroxy-octanoyl)-l-homoserine lactone, N-(3-hydroxy-decanoyl)-l-homoserine lactone and N-(3-hydroxy-dodecanoyl)-l-homoserine lactone (3-hydroxy-C8-HSL, 3-hydroxy-C10-HSL and 3-oxo-C12-HSL, respectively) were also detected by HPLC-HRMS analysis from in vitro cultures. Most studies of quorum sensing (QS) systems have been conducted in vitro, the purpose of our study was to determine if the same acylated homoserine lactones were produced in vivo during infection. Extracts from infected fish were purified using several solid phase extraction strategies to allow chromatographic detection and separation by both TLC and HLPC-HRMS. 3-oxo-C10-HSL and 3-hydroxy-C6-HSL were detected in organs from fish dying from vibriosis, however, compared to in vitro culturing where 3-oxo-C10-HSL is the dominant molecule, 3-hydroxy-C6-HSL was prominent in the infected fish tissues. Hence, the balance between the QS systems may be different during infection compared to in vitro cultures. For future studies of QS systems and the possible specific interference with expression of virulence factors, in vitro cultures should be optimised to reflect the in vivo situation.

Ämnesord och genrebeteckningar

4-Butyrolactone/*analogs & derivatives/analysis/isolation & purification
Adaptation; Physiological
Animals
Chromatography; High Pressure Liquid
Chromatography; Thin Layer
Fish Diseases/microbiology
Genes; Bacterial
Iron/metabolism
Mass Spectrometry
Oncorhynchus mykiss
Serotyping
Sodium Chloride/metabolism
Temperature
Vibrio/*chemistry/classification/growth & development/physiology
Vibrio Infections/microbiology/veterinary

Biuppslag (personer, institutioner, konferenser, titlar ...)

Nielsen, Kristian Fog (författare)
Milton, Debra LUmeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)(Swepub:umu)demi0001 (författare)
Larsen, Jens Laurits (författare)
Gram, Lone (författare)
Umeå universitetInstitutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet) (creator_code:org_t)

Sammanhörande titlar

Ingår i:Syst Appl Microbiol29:6, s. 433-450723-2020

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Av författaren/redakt...: Buchholtz, Chris ...; Nielsen, Kristia ...; Milton, Debra L; Larsen, Jens Lau ...; Gram, Lone

Artiklar i publikationen: Syst Appl Microb ...

Av lärosätet: Umeå universitet

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