A modified protein marker panel to identify four consensus molecular subtypes in colorectal cancer using immunohistochemistry

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: WFRF:(Ling Agnes) > A modified protein ...

Details
MARC

Li, XingruUmeå universitet,Patologi (author)

A modified protein marker panel to identify four consensus molecular subtypes in colorectal cancer using immunohistochemistry

Article/chapterEnglish2021

Publisher, publication year, extent ...

Elsevier,2021
electronicrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:umu-181731
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-181731URI
https://doi.org/10.1016/j.prp.2021.153379DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Colorectal cancer (CRC) is a heterogeneous disease with different genetic and molecular backgrounds, leading to a diverse patient prognosis and treatment response. Four consensus molecular subtypes (CMS 1–4) have recently been proposed based on transcriptome profiling. A clinically practical immunohistochemistry (IHC) based CMS classifier consisting of the four markers FRMD6, ZEB1, HTR2B, and CDX2 was then demonstrated. However, the IHC-CMS classifier did not distinguish between CMS2 and CMS3 tumours. In this study, we have applied the proposed transcriptome based and IHC-based CMS classifiers in a CRC cohort of 65 patients and found a concordance of 77.5 %. Further, we modified the IHC-CMS classifier by analysing the differentially expressed genes between CMS2 and CMS3 tumours using RNA-sequencing data from the TCGA dataset. The result showed that WNT signalling was among the most upregulated pathways in CMS2 tumours, and the expression level of CTNNB1 (encoding β-catenin), a WNT pathway hallmark, was significantly upregulated (P = 1.15 × 10−6). We therefore introduced nuclear β-catenin staining to the IHC-CMS classifier. Using the modified classifier in our cohort, we found a 71.4 % concordance between the IHC and RNA-sequencing based CMS classifiers. Moreover, β-catenin staining could classify 16 out of the 19 CMS2/3 tumours into CMS2 or CMS3, thereby showing an 84.2 % concordance with the RNA-sequencing-based classifier. In conclusion, we evaluated CMS classifiers based on transcriptome and IHC analysis. We present a modified IHC panel that categorizes CRC tumours into the four CMS groups. To our knowledge, this is the first study using IHC to identify all four CMS groups.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Cancer och onkologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Cancer and Oncology hsv//eng
Colorectal cancer
Consensus molecular subtypes
Immunohistochemistry
Protein marker panel
β-catenin

Added entries (persons, corporate bodies, meetings, titles ...)

Larsson, PärUmeå universitet,Patologi(Swepub:umu)pala0006 (author)
Ljuslinder, Ingrid,1968-Umeå universitet,Onkologi(Swepub:umu)inlj0001 (author)
Ling, AgnesUmeå universitet,Patologi(Swepub:umu)agslig01 (author)
Löfgren Burström, AnnaUmeå universitet,Patologi(Swepub:umu)anlo0002 (author)
Zingmark, Carl,1975-Umeå universitet,Patologi(Swepub:umu)cazi0001 (author)
Edin, SofiaUmeå universitet,Patologi(Swepub:umu)soed0001 (author)
Palmqvist, RichardUmeå universitet,Patologi(Swepub:umu)ripa0001 (author)
Umeå universitetPatologi (creator_code:org_t)

Related titles

In:Pathology, Research and Practice: Elsevier2200344-03381618-0631

Internet link

Find in a library

Pathology, Research and Practice (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: Li, Xingru; Larsson, Pär; Ljuslinder, Ingr ...; Ling, Agnes; Löfgren Burström ...; Zingmark, Carl, ...; show more...; Edin, Sofia; Palmqvist, Richa ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cancer and Oncol ...

Articles in the publication: Pathology, Resea ...

By the university: Umeå University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se