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Combining in silico...
Combining in silico and in vitro models to inform cell seeding strategies in tissue engineering
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Coy, R. (författare)
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Al-Badri, G. (författare)
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Kayal, C. (författare)
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O'Rourke, C (författare)
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- Kingham, Paul J. (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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Phillips, J. B. (författare)
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Shipley, R. J. (författare)
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(creator_code:org_t)
- 2020-03-25
- 2020
- Engelska.
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Ingår i: Journal of the Royal Society Interface. - : The Royal Society Publishing. - 1742-5689 .- 1742-5662. ; 17:164
- Relaterad länk:
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https://royalsociety...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The seeding density of therapeutic cells in engineered tissue impacts both cell survival and vascularization. Excessively high seeded cell densities can result in increased death and thus waste of valuable cells, whereas lower seeded cell densities may not provide sufficient support for the tissue in vivo, reducing efficacy. Additionally, the production of growth factors by therapeutic cells in low oxygen environments offers a way of generating growth factor gradients, which are important for vascularization, but hypoxia can also induce unwanted levels of cell death. This is a complex problem that lends itself to a combination of computational modelling and experimentation. Here, we present a spatio-temporal mathematical model parametrized using in vitro data capable of simulating the interactions between a therapeutic cell population, oxygen concentrations and vascular endothelial growth factor (VEGF) concentrations in engineered tissues. Simulations of collagen nerve repair constructs suggest that specific seeded cell densities and non-uniform spatial distributions of seeded cells could enhance cell survival and the generation of VEGF gradients. These predictions can now be tested using targeted experiments.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- interdisciplinary
- mathematical modelling
- nerve
- oxygen
- tissue engineering
- Human Anatomy
- anatomi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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