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  • Bortz, Robert H.Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States (författare)

Single-Dilution COVID-19 Antibody Test with Qualitative and Quantitative Readouts

  • Artikel/kapitelEngelska2021

Förlag, utgivningsår, omfång ...

  • American Society for Microbiology,2021
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:umu-183128
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-183128URI
  • https://doi.org/10.1128/mSphere.00224-21DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • The coronavirus disease 2019 (COVID-19) global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to place an immense burden on societies and health care systems. A key component of COVID-19 control efforts is serological testing to determine the community prevalence of SARS-CoV-2 exposure and quantify individual immune responses to prior SARS-CoV-2 infection or vaccination. Here, we describe a laboratory-developed antibody test that uses readily available research-grade reagents to detect SARS-CoV-2 exposure in patient blood samples with high sensitivity and specificity. We further show that this sensitive test affords the estimation of viral spike-specific IgG titers from a single sample measurement, thereby providing a simple and scalable method to measure the strength of an individual's immune response. The accuracy, adaptability, and cost-effectiveness of this test make it an excellent option for clinical deployment in the ongoing COVID-19 pandemic.IMPORTANCE Serological surveillance has become an important public health tool during the COVID-19 pandemic. Detection of protective antibodies and seroconversion after SARS-CoV-2 infection or vaccination can help guide patient care plans and public health policies. Serology tests can detect antibodies against past infections; consequently, they can help overcome the shortcomings of molecular tests, which can detect only active infections. This is important, especially when considering that many COVID-19 patients are asymptomatic. In this study, we describe an enzyme-linked immunosorbent assay (ELISA)-based qualitative and quantitative serology test developed to measure IgG and IgA antibodies against the SARS-CoV-2 spike glycoprotein. The test can be deployed using commonly available laboratory reagents and equipment and displays high specificity and sensitivity. Furthermore, we demonstrate that IgG titers in patient samples can be estimated from a single measurement, enabling the assay's use in high-throughput clinical environments.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Florez, CatalinaDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY, United States (författare)
  • Laudermilch, EthanDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Wirchnianski, Ariel S.Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Lasso, GorkaDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Malonis, Ryan J.Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Georgiev, George I.Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Vergnolle, OliviaDepartment of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Herrera, Natalia G.Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Morano, Nicholas C.Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Campbell, Sean T.Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States (författare)
  • Orner, Erika P.Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States (författare)
  • Mengotto, AmandaMontefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Dieterle, M. EugeniaDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Fels, J. MaximilianDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Haslwanter, DeniseDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Jangra, Rohit K.Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Celikgil, AlevDepartment of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Kimmel, DuncanMontefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Lee, James H.Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Mariano, Margarette C.Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Nakouzi, AntonioDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Quiroz, JoseMontefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Rivera, JohannaDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Szymczak, Wendy A.Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States (författare)
  • Tong, KarenDepartment of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Barnhill, JasonDepartment of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY, United States (författare)
  • Forsell, Mattias N. E.Umeå universitet,Institutionen för klinisk mikrobiologi(Swepub:umu)mafo0173 (författare)
  • Ahlm, Clas,1956-Umeå universitet,Institutionen för klinisk mikrobiologi(Swepub:umu)clah0001 (författare)
  • Stein, Daniel T.Montefiore Medical Center, NY, Bronx, United States; Division of Endocrinology and Diabetes, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Pirofski, Liise-AnneDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Goldstein, D YitzchakDepartment of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States (författare)
  • Garforth, Scott J.Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Almo, Steven C.Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Daily, Johanna P.Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Prystowsky, Michael B.Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States (författare)
  • Faix, James D.Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States (författare)
  • Fox, Amy S.Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States (författare)
  • Weiss, Louis M.Montefiore Medical Center, NY, Bronx, United States; Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Lai, Jonathan R.Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Chandran, KartikDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States (författare)
  • Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United StatesDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY, United States (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:mSphere: American Society for Microbiology6:22379-5042

Internetlänk

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  • mSphere (Sök värdpublikationen i LIBRIS)

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