Initial development towards in vitro toxicity assay for lactate dehydrogenase using surface plasmon resonance

• Work with initial development towards in vitro toxicity assay for lactate dehydrogenase (LDH) using surface plasmon resonance is described. LDH is one of the important versatile biomarkers in in vitro hepatotoxicity. In this report LDH is detected by surface plasmon resonance using antibodies directly immobilized to the sensor surface. The assay is further extended with protein G immobilized to the surface to capture the antibody ligand. This has the advantage of simultaneously and on site purify and orient antibody ligand.

Nyckelord

Lactate dehydrogenase

surface plasmon resonance

in vitro toxicity

biosensor

protein G

Publikations- och innehållstyp

vet (ämneskategori)

ovr (ämneskategori)