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Effects of x-ray free-electron laser pulse intensity on the Mn K β 1,3x-ray emission spectrum in photosystem II - A case study for metalloprotein crystals and solutions

Fransson, Thomas (författare)
KTH Royal Institute of Technology
Alonso-Mori, Roberto (författare)
Linac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States,Stanford Linear Accelerator Center (SLAC)
Chatterjee, Ruchira (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States
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Cheah, Mun Hon (författare)
Uppsala University
Ibrahim, Mohamed (författare)
Humboldt-Universität zu Berlin, Department of Biology, Berlin, Germany
Hussein, Rana (författare)
Humboldt-Universität zu Berlin, Department of Biology, Berlin, Germany
Zhang, Miao (författare)
Humboldt-Universität zu Berlin, Department of Biology, Berlin, Germany
Fuller, Franklin (författare)
Linac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States,Stanford Linear Accelerator Center (SLAC)
Gul, Sheraz (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States
Kim, In-Sik (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States
Simon, Philipp S. (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States,Stanford Linear Accelerator Center (SLAC)
Bogacz, Isabel (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States
Makita, Hiroki (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States
de Lichtenberg, Casper (författare)
Uppsala University,Umeå University,Umeå universitet,Kemiska institutionen,Department of Chemistry - Ångström Laboratory, Molecular Biomimetics, Uppsala University, Uppsala, Sweden; Section of Biomolecular Sciences, Department of Biology, University of Copenhagen, Copenhagen N, Denmark
Song, Sanghoon (författare)
Linac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States
Batyuk, Alexander (författare)
Linac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States,Stanford Linear Accelerator Center (SLAC)
Sokaras, Dimosthenis (författare)
Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, CA, Menlo Park, United States,Stanford Linear Accelerator Center (SLAC)
Massad, Ramzi (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States
Doyle, Margaret (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States
Britz, Alexander (författare)
Linac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States; Stanford PULSE Institute, SLAC National Accelerator Laboratory, CA, Menlo Park, United States,Stanford Linear Accelerator Center (SLAC)
Weninger, Clemens (författare)
Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory
Zouni, Athina (författare)
Humboldt-Universität zu Berlin, Department of Biology, Berlin, Germany
Messinger, Johannes, 1963- (författare)
Uppsala University,Umeå University,Umeå universitet,Kemiska institutionen,Department of Chemistry - Ångström Laboratory, Molecular Biomimetics, Uppsala University, Uppsala, Sweden
Yachandra, Vittal K. (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States
Yano, Junko (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States
Kern, Jan (författare)
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States
Bergmann, Uwe (författare)
Department of Physics, University of Wisconsin-Madison, WI, Madison, United States
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 (creator_code:org_t)
American Institute of Physics (AIP), 2021
2021
Engelska.
Ingår i: Structural Dynamics. - : American Institute of Physics (AIP). - 2329-7778. ; 8:6
Relaterad länk:
https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
https://aca.scitatio...
http://dx.doi.org/10... (free)
https://urn.kb.se/re...
https://doi.org/10.1...
https://lup.lub.lu.s...
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • In the last ten years, x-ray free-electron lasers (XFELs) have been successfully employed to characterize metalloproteins at room temperature using various techniques including x-ray diffraction, scattering, and spectroscopy. The approach has been to outrun the radiation damage by using femtosecond (fs) x-ray pulses. An example of an important and damage sensitive active metal center is the Mn4CaO5 cluster in photosystem II (PS II), the catalytic site of photosynthetic water oxidation. The combination of serial femtosecond x-ray crystallography and Kβ x-ray emission spectroscopy (XES) has proven to be a powerful multimodal approach for simultaneously probing the overall protein structure and the electronic state of the Mn4CaO5 cluster throughout the catalytic (Kok) cycle. As the observed spectral changes in the Mn4CaO5 cluster are very subtle, it is critical to consider the potential effects of the intense XFEL pulses on the Kβ XES signal. We report here a systematic study of the effects of XFEL peak power, beam focus, and dose on the Mn Kβ1,3 XES spectra in PS II over a wide range of pulse parameters collected over seven different experimental runs using both microcrystal and solution PS II samples. Our findings show that for beam intensities ranging from ∼5 × 1015 to 5 × 1017 W/cm2 at a pulse length of ∼35 fs, the spectral effects are small compared to those observed between S-states in the Kok cycle. Our results provide a benchmark for other XFEL-based XES studies on metalloproteins, confirming the viability of this approach.

Ämnesord

NATURVETENSKAP  -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
NATURAL SCIENCES  -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)

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