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The barrier functio...
The barrier functions of crude cervical mucus plugs against HIV-1 infection in the context of cell-free and cell-to-cell transmission
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- Mhlekude, Baxolele (författare)
- University of Cape Town, Department of Surgery, Groote Schuur Hospital, Observatory, South Africa; TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Hannover Medical School and the Helmholtz Centre for Infection Research, Institute of Experimental Virology, Hannover; Charité - Universitätsmedizin Berlin, Institute of Virology, Charité Campus Mitte; Berlin Institute of Health, Berlin, Germany
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- Lenman, Annasara (författare)
- TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Hannover Medical School and the Helmholtz Centre for Infection Research, Institute of Experimental Virology, Hannover
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- Sidoyi, Phikolomzi (författare)
- Faculty of Health Sciences, School of Medicine, Walter Sisulu University, Mthatha, South Africa
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- Joseph, Jim (författare)
- Department of Human Biology, Walter Sisulu University, Mthatha, South Africa
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- Kruppa, Jochen (författare)
- Charité - Universitätsmedizin Berlin, Institut für Biometrie und Klinische Epidemiologie, Charité Campus Mitte, Berlin, Germany
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- Businge, Charles Bitamazire (författare)
- Department of Obstetrics and Gynaecology, Walter Sisulu University/Nelson Mandela Academic Hospital
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- Mdaka, Mana Lungisa (författare)
- Department of Obstetrics and Gynaecology, Walter Sisulu University/Nelson Mandela Academic Hospital
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- Konietschke, Frank (författare)
- Berlin Institute of Health, Berlin, Germany; Charité - Universitätsmedizin Berlin, Institut für Biometrie und Klinische Epidemiologie, Charité Campus Mitte, Berlin, Germany
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- Pich, Andreas (författare)
- Hannover Medical School, Institute of Toxicology, Core Facility Proteomics, Hannover, Germany
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- Gerold, Gisa, 1979- (författare)
- Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Avdelningen för virologi,TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Hannover Medical School and the Helmholtz Centre for Infection Research, Institute of Experimental Virology, Hannover; Department of Biochemistry, University of Veterinary Medicine Hannover, Hanover, Germany
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- Goffinet, Christine (författare)
- TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Hannover Medical School and the Helmholtz Centre for Infection Research, Institute of Experimental Virology, Hannover; Charité - Universitätsmedizin Berlin, Institute of Virology, Charité Campus Mitte; Berlin Institute of Health, Berlin, Germany
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- Mall, Anwar Suleman (författare)
- University of Cape Town, Department of Surgery, Groote Schuur Hospital, Observatory, South Africa
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(creator_code:org_t)
- Wolters Kluwer, 2021
- 2021
- Engelska.
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Ingår i: AIDS. - : Wolters Kluwer. - 0269-9370 .- 1473-5571. ; 35:13, s. 2105-2117
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- OBJECTIVE: The cervical mucus plugs are enriched with proteins of known immunological functions. We aimed to characterize the anti-HIV-1 activity of the cervical mucus plugs against a panel of different HIV-1 strains in the contexts of cell-free and cell-associated virus.DESIGN: A cohort of consenting HIV-1-negative and HIV-1-positive pregnant women in labour was recruited from Mthatha General Hospital in the Eastern Cape province of South Africa, from whom the cervical mucus plugs were collected in 6 M guanidinium chloride with protease inhibitors and transported to our laboratories at -80 °C.METHODS: Samples were centrifuged to remove insoluble material and dialysed before freeze--drying and subjecting them to the cell viability assays. The antiviral activities of the samples were studied using luminometric reporter assays and flow cytometry. Time-of-addition and BlaM-Vpr virus-cell fusion assays were used to pin-point the antiviral mechanisms of the cervical mucus plugs, before proteomic profiling using liquid chromatography-tandem mass spectrometry.RESULTS: The proteinaceous fraction of the cervical mucus plugs exhibited anti-HIV-1 activity with inter-individual variations and some degree of specificity among different HIV-1 strains. Cell-associated HIV-1 was less susceptible to inhibition by the potent samples whenever compared with the cell-free HIV-1. The samples with high antiviral potency exhibited a distinct proteomic profile when compared with the less potent samples.CONCLUSION: The crude cervical mucus plugs exhibit anti-HIV-1 activity, which is defined by a specific proteomic profile.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
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Mhlekude, Baxole ...
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Lenman, Annasara
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Sidoyi, Phikolom ...
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Joseph, Jim
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Kruppa, Jochen
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Businge, Charles ...
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Mdaka, Mana Lung ...
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Konietschke, Fra ...
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Pich, Andreas
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Gerold, Gisa, 19 ...
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Goffinet, Christ ...
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Mall, Anwar Sule ...
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