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RelA-SpoT Homolog t...
RelA-SpoT Homolog toxins pyrophosphorylate the CCA end of tRNA to inhibit protein synthesis
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- Kurata, Tatsuaki (författare)
- Lund University,Lunds universitet,Molekylär enzymologi,Forskargrupper vid Lunds universitet,Molecular Enzymology,Lund University Research Groups
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- Brodiazhenko, Tetiana (författare)
- University of Tartu, Institute of Technology, Tartu, Estonia
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- Alves Oliveira, Sofia Raquel (författare)
- University of Tartu, Institute of Technology, Tartu, Estonia
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- Roghanian, Mohammad (författare)
- Umeå University,Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark
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- Sakaguchi, Yuriko (författare)
- Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo, Japan,Tokyo Denki University
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- Turnbull, Kathryn Jane (författare)
- Umeå University,Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark
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- Bulvas, Ondřej (författare)
- Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., Flemingovonam. 2, Prague 6, Czech Republic
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- Takada, Hiraku (författare)
- Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo, Motoyama, Kita-ku, Kyoto, Japan
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- Tamman, Hedvig (författare)
- Cellular and Molecular Microbiology (CM2), Faculté des Sciences, Université Libre de Bruxelles (ULB), Campus La Plaine, Building BC, Room 1C4203, Boulevard du Triomphe, Brussels, Belgium
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- Ainelo, Andres (författare)
- Cellular and Molecular Microbiology (CM2), Faculté des Sciences, Université Libre de Bruxelles (ULB), Campus La Plaine, Building BC, Room 1C4203, Boulevard du Triomphe, Brussels, Belgium
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- Pohl, Radek (författare)
- Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., Flemingovonam. 2, Prague 6, Czech Republic
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- Rejman, Dominik (författare)
- Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., Flemingovonam. 2, Prague 6, Czech Republic
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- Tenson, Tanel (författare)
- University of Tartu, Institute of Technology, Tartu, Estonia
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- Suzuki, Tsutomu (författare)
- Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo, Japan,Tokyo Denki University
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- Garcia-Pino, Abel (författare)
- Cellular and Molecular Microbiology (CM2), Faculté des Sciences, Université Libre de Bruxelles (ULB), Campus La Plaine, Building BC, Room 1C4203, Boulevard du Triomphe, Brussels, Belgium; WELBIO, Avenue Hippocrate 75, Brussels, Belgium
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- Atkinson, Gemma Catherine (författare)
- Lund University,Lunds universitet,Proteinevolution,Forskargrupper vid Lunds universitet,Protein Evolution,Lund University Research Groups
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- Hauryliuk, Vasili, 1980- (författare)
- Umeå University,Lund University,Lunds universitet,Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Experimental Medical Science, Lund University, Lund, Sweden; University of Tartu, Institute of Technology, Tartu, Estonia,Molekylär enzymologi,Forskargrupper vid Lunds universitet,Molecular Enzymology,Lund University Research Groups
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(creator_code:org_t)
- Cell Press, 2021
- 2021
- Engelska.
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Ingår i: Molecular Cell. - : Cell Press. - 1097-2765 .- 1097-4164. ; 81:15, s. 3160-3170.e9
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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http://www.cell.com/...
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- RelA-SpoT Homolog (RSH) enzymes control bacterial physiology through synthesis and degradation of the nucleotide alarmone (p)ppGpp. We recently discovered multiple families of small alarmone synthetase (SAS) RSH acting as toxins of toxin-antitoxin (TA) modules, with the FaRel subfamily of toxSAS abrogating bacterial growth by producing an analog of (p)ppGpp, (pp)pApp. Here we probe the mechanism of growth arrest used by four experimentally unexplored subfamilies of toxSAS: FaRel2, PhRel, PhRel2, and CapRel. Surprisingly, all these toxins specifically inhibit protein synthesis. To do so, they transfer a pyrophosphate moiety from ATP to the tRNA 3′ CCA. The modification inhibits both tRNA aminoacylation and the sensing of cellular amino acid starvation by the ribosome-associated RSH RelA. Conversely, we show that some small alarmone hydrolase (SAH) RSH enzymes can reverse the pyrophosphorylation of tRNA to counter the growth inhibition by toxSAS. Collectively, we establish RSHs as RNA-modifying enzymes.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- (p)ppApp
- (p)ppGpp
- RelA-SpoT Homolog
- ribosome
- SAH
- SAS
- toxin-antitoxin
- toxSAS
- translation
- tRNA modification
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Kurata, Tatsuaki
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Brodiazhenko, Te ...
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Alves Oliveira, ...
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Roghanian, Moham ...
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Sakaguchi, Yurik ...
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Turnbull, Kathry ...
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visa fler...
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Bulvas, Ondřej
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Takada, Hiraku
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Tamman, Hedvig
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Ainelo, Andres
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Pohl, Radek
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Rejman, Dominik
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Tenson, Tanel
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Suzuki, Tsutomu
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Garcia-Pino, Abe ...
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Atkinson, Gemma ...
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Hauryliuk, Vasil ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
- Artiklar i publikationen
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Molecular Cell
- Av lärosätet
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Umeå universitet
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Lunds universitet