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pH induced changes ...
pH induced changes in Raman, UV-Vis absorbance, and fluorescence spectra of dipicolinic acid (DPA)
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- Malyshev, Dmitry (författare)
- Umeå universitet,Institutionen för fysik,Umeå Univ, Dept Phys, S-90187 Umeå, Sweden
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- Öberg, Rasmus (författare)
- Umeå universitet,Institutionen för fysik,Umeå Univ, Dept Phys, S-90187 Umeå, Sweden
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- Landström, Lars (författare)
- Swedish Def Res Agcy FOI, Umeå, Sweden
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- Andersson, Per Ola (författare)
- Uppsala universitet,Tillämpad materialvetenskap,Institutionen för teknikvetenskaper,Swedish Def Res Agcy FOI, Umeå, Sweden
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- Dahlberg, Tobias (författare)
- Umeå universitet,Institutionen för fysik,Umeå Univ, Dept Phys, S-90187 Umeå, Sweden
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- Andersson, Magnus (författare)
- Umeå universitet,Institutionen för fysik,Umeå Centre for Microbial Research (UCMR),The Biophysics and Biophotonics group,Umeå Univ, Dept Phys, S-90187 Umeå, Sweden.;Umeå Ctr Microbial Res UCMR, Umeå, Sweden.
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(creator_code:org_t)
- Elsevier, 2022
- 2022
- Engelska.
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Ingår i: Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy. - : Elsevier. - 1386-1425 .- 1873-3557. ; 271
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Abstract
Ämnesord
Stäng
- Dipicolinic acid (DPA) is an essential component for the protection of DNA in bacterial endospores and is often used as a biomarker for spore detection. Depending upon the pH of the solution, DPA exists in different ionic forms. Therefore, it is important to understand how these ionic forms influence spectroscopic response. In this work, we characterize Raman and absorption spectra of DPA in a pH range of 2.0–10.5. We show that the ring breathing mode Raman peak of DPA shifts from 1003 cm−1 to 1017 cm−1 and then to 1000 cm−1 as pH increases from 2 to 5. The relative peak intensities related to the different ionic forms of DPA are used to experimentally derive the pKa values (2.3 and 4.8). We observe using UV–vis spectroscopy that the changes in the absorption spectrum of DPA as a function of pH correlate with the changes observed in Raman spectroscopy, and the same pKa values are verified. Lastly, using fluorescence spectroscopy and exciting a DPA solution at between 210–330 nm, we observe a shift in fluorescence emission from 375 nm to 425 nm between pH 2 and pH 6 when exciting at 320 nm. Our work shows that the different spectral responses from the three ionic forms of DPA may have to be taken into account in, e.g., spectral analysis and for detection applications.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- NATURVETENSKAP -- Kemi -- Oorganisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Inorganic Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Nyckelord
- Bacterial spores
- DPA
- Biomarker
- Raman spectra
- UV–vis absorption spectra
- Fluorescence spectra
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- art (ämneskategori)
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