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Novel two-component...
Novel two-component system-like elements reveal functional domains associated with restriction-modification systems and paramorc atpases in bacteria
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- Bellieny-Rabelo, Daniel (författare)
- Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Gauteng, South Africa; Forestry and Agricultural Biotechnology Institute, University of Pretoria, Gauteng, South Africa
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- Pretorius, Willem J. S. (författare)
- Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Gauteng, South Africa; Forestry and Agricultural Biotechnology Institute, University of Pretoria, Gauteng, South Africa
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- Moleleki, Lucy N. (författare)
- Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Gauteng, South Africa; Forestry and Agricultural Biotechnology Institute, University of Pretoria, Gauteng, South Africa
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(creator_code:org_t)
- 2021-02-10
- 2021
- Engelska.
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Ingår i: Genome Biology and Evolution. - : Oxford University Press. - 1759-6653. ; 13:3
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Abstract
Ämnesord
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- Two-component systems (TCS) are important types of machinery allowing for efficient signal recognition and transmission in bacterial cells. The majority of TCSs utilized by bacteria is composed of a sensor histidine kinase (HK) and a cognate response regulator (RR). In the present study, we report two newly predicted protein domains-both to be included in the next release of the Pfam database: Response_reg_2 (PF19192) and HEF_HK (PF19191)-in bacteria which exhibit high structural similarity, respectively, with typical domains of RRs and HKs. Additionally, the genes encoding for the novel predicted domains exhibit a 91.6% linkage observed across 644 genomic regions recovered from 628 different bacterial strains. The remarkable adjacent colocalization between genes carrying Response_reg_2 and HEF_HK in addition to their conserved structural features, which are highly similar to those from well-known HKs and RRs, raises the possibility of Response_reg_2 and HEF_HK constituting a new TCS in bacteria. The genomic regions in which these predicted two-component systems-like are located additionally exhibit an overrepresented presence of restriction-modification (R-M) systems especially the type II R-M. Among these, there is a conspicuous presence of C-5 cytosine-specific DNA methylases which may indicate a functional association with the newly discovered domains. The solid presence of R-M systems and the presence of the GHKL family domain HATPase_c_3 across most of the HEF_HK-containing genes are also indicative that these genes are evolutionarily related to the paraMORC family of ATPases.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
Nyckelord
- two-component system
- MORC
- GHKL ATPases
- DNA modification methylases
- DNA restriction-modification enzymes
- histidine kinase
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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