Optimization of biofuel production by molecular characterization of ligninase enzyme isolated from wood rot fungi in Pushpagiri and Brahmagiri Hills in Karnataka by using RAPD molecular markers

• Wood rot fungi are one of the major degraders in the biosphere that help in degrading most of the plant origin polymers like cellulose, hemicellulose and lignin. Lignin is the second most abundant aromatic compound found in plant cell that holds up cellulose and hemicellulose. There is a lot of emphasis that is being put on the fungal degradation of lignin using wood and other lignocellulosic as a renewable source in the production of chemicals, paper products, feeds and fuels and the use of fungi as one of the most potent sources of degrading organisms. In the present study, screening for lignin degrading enzymes were done with 132 isolates and the maximum enzyme producing strains of 10 wood rot fungi samples were taken for molecular characterization using RAPD molecular markers.Isolation of genomic DNA of the 10 wood rot fungi samples was done using phenolchloroform method and quantified on agarose gel. The obtained genomic DNA was further subjected tocharacterization using RAPD-PCR method with 06 random primers OPA2, OPA5, OPA7, OPA8, OPD3 and OPC2. The amplified PCR products were analyzed by Agarose Gel Electrophoresis and were observed under UV Transilluminator. Analysis of the base pairs of the bands was done by Bio-Rad Gel Doc system.A total of 172 fragments were generated in the 10 isolates with 6 primers. Dendrogram analyses of the gels were done which gave a close relation of each DNA samples. The results indicate that some strains were genetically more similar and few diverse. The current study shows samples 30, 40, 41, 113 and 124 are genetically closely related.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Ligninase enzyme

Molecular markers

PCR

RAPD

Wood rot fungi

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