Search: WFRF:(Knight Stefan) >
Archaic chaperone-u...
Archaic chaperone-usher pili self-secrete into superelastic zigzag springs
-
- Pakharukova, Natalia (author)
- Joint Biotechnology Laboratory, MediCity, Faculty of Medicine, University of Turku, Turku, Finland,Univ Turku, Fac Med, Joint Biotechnol Lab, MediCity, Turku, Finland.
-
- Malmi, Henri (author)
- Joint Biotechnology Laboratory, MediCity, Faculty of Medicine, University of Turku, Turku, Finland,Univ Turku, Fac Med, Joint Biotechnol Lab, MediCity, Turku, Finland.
-
- Tuittila, Minna (author)
- Joint Biotechnology Laboratory, MediCity, Faculty of Medicine, University of Turku, Turku, Finland,Univ Turku, Fac Med, Joint Biotechnol Lab, MediCity, Turku, Finland.
-
show more...
-
- Dahlberg, Tobias, 1990- (author)
- Umeå universitet,Institutionen för fysik,Umeå Centre for Microbial Research (UCMR),The Biophysics and Biophotonics group,Umeå Univ, Umeå Ctr Microbial Res UCMR, Dept Phys, Umeå, Sweden.
-
- Ghosal, Debnath (author)
- Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, USA,CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA.;Univ Melbourne, Div Med Dent & Hearth Sci, Parkville, Vic, Australia.
-
- Chang, Yi-Wei (author)
- Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, USA,CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA.;Univ Penn, Dept Biochem & Biophys, Perelman Sch Med, Philadelphia, PA 19104 USA.
-
- Myint, Si Lhyam (author)
- Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten),Umeå Centre for Microbial Research (UCMR),Umeå Univ, Umeå Ctr Microbial Res UCMR, Dept Mol Biol, Lab Mol Infect Med Sweden MIMS, Umeå, Sweden.
-
- Paavilainen, Sari (author)
- Joint Biotechnology Laboratory, MediCity, Faculty of Medicine, University of Turku, Turku, Finland,Univ Turku, Fac Med, Joint Biotechnol Lab, MediCity, Turku, Finland.
-
- Knight, Stefan D. (author)
- Uppsala universitet,Strukturbiologi
-
- Lamminmäki, Urpo (author)
- Department of Biochemistry, University of Turku, Turku, Finland,Univ Turku, Dept Life Technol, Turku, Finland.
-
- Uhlin, Bernt Eric (author)
- Umeå universitet,Umeå Centre for Microbial Research (UCMR),Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten),Umeå Univ, Umeå Ctr Microbial Res UCMR, Dept Mol Biol, Lab Mol Infect Med Sweden MIMS, Umeå, Sweden.
-
- Andersson, Magnus (author)
- Umeå universitet,Institutionen för fysik,Umeå Centre for Microbial Research (UCMR),The Biophysics and Biophotonics group,Umeå Univ, Umeå Ctr Microbial Res UCMR, Dept Phys, Umeå, Sweden.
-
- Jensen, Grant (author)
- Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, USA,CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA.
-
- Zavialov, Anton V. (author)
- Joint Biotechnology Laboratory, MediCity, Faculty of Medicine, University of Turku, Turku, Finland,Univ Turku, Fac Med, Joint Biotechnol Lab, MediCity, Turku, Finland.
-
show less...
-
Joint Biotechnology Laboratory, MediCity, Faculty of Medicine, University of Turku, Turku, Finland Univ Turku, Fac Med, Joint Biotechnol Lab, MediCity, Turku, Finland (creator_code:org_t)
- 2022-07-19
- 2022
- English.
-
In: Nature. - : Nature Publishing Group. - 0028-0836 .- 1476-4687. ; 609:7926, s. 335-340
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://umu.diva-por... (primary) (Raw object)
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Adhesive pili assembled via the chaperone-usher pathway (CUP) are hair-like appendages that mediate host tissue colonization and biofilm formation of Gram-negative bacteria 1-3. Archaic CUP pili, the most diverse and widespread CUP adhesins, are promising vaccine and drug targets due to their prevalence in the most troublesome multidrug-resistant (MDR) pathogens 1,4,5. However, their architecture and assembly-secretion process remain unknown. Here, we present the 3.4 Å resolution cryo-electron microscopy structure of the prototypical archaic Csu pilus that mediates biofilm formation of Acinetobacter baumannii, a notorious MDR nosocomial pathogen. In contrast to the thick helical tubes of the classical type 1 and P pili, archaic pili assemble into a conceptually novel ultrathin zigzag architecture secured by an elegant clinch mechanism. The molecular clinch provides the pilus with high mechanical stability as well as superelasticity, a property observed now for the first time in biomolecules, while enabling a more economical and faster pilus production. Furthermore, we demonstrate that clinch formation at the cell surface drives pilus secretion through the outer membrane. These findings suggest that clinch-formation inhibitors might represent a new strategy to fight MDR bacterial infections.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Keyword
- mikrobiologi
- Microbiology
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
Nature
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Pakharukova, Nat ...
-
Malmi, Henri
-
Tuittila, Minna
-
Dahlberg, Tobias ...
-
Ghosal, Debnath
-
Chang, Yi-Wei
-
show more...
-
Myint, Si Lhyam
-
Paavilainen, Sar ...
-
Knight, Stefan D ...
-
Lamminmäki, Urpo
-
Uhlin, Bernt Eri ...
-
Andersson, Magnu ...
-
Jensen, Grant
-
Zavialov, Anton ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Microbiology in ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Physical Science ...
-
and Other Physics To ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Structural Biolo ...
- Articles in the publication
-
Nature
- By the university
-
Umeå University
-
Uppsala University