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Efficacy of Nerve-D...
Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization
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- Kumar Kuna, Vijay (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Lundgren, André (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Handkirurgi
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- Anerillas, Luis Oliveros (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Kelk, Peyman (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Institutionen för odontologi
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- Brohlin, Maria, 1966- (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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- Wiberg, Mikael (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Handkirurgi
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- Kingham, Paul J. (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Novikova, Ludmila N. (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Andersson, Gustav (author)
- Umeå universitet,Handkirurgi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Institutionen för integrativ medicinsk biologi (IMB)
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- Novikov, Lev N. (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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(creator_code:org_t)
- 2022-08-06
- 2022
- English.
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In: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 23:15
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https://doi.org/10.3...
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https://umu.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- Injuries to large peripheral nerves are often associated with tissue defects and require reconstruction using autologous nerve grafts, which have limited availability and result in donor site morbidity. Peripheral nerve-derived hydrogels could potentially supplement or even replace these grafts. In this study, three decellularization protocols based on the ionic detergents sodium dodecyl sulfate (P1) and sodium deoxycholate (P2), or the organic solvent tri-n-butyl phosphate (P3), were used to prepare hydrogels. All protocols resulted in significantly decreased amounts of genomic DNA, but the P2 hydrogel showed the best preservation of extracellular matrix proteins, cytokines, and chemokines, and reduced levels of sulfated glycosaminoglycans. In vitro P1 and P2 hydrogels supported Schwann cell viability, secretion of VEGF, and neurite outgrowth. Surgical repair of a 10 mm-long rat sciatic nerve gap was performed by implantation of tubular polycaprolactone conduits filled with hydrogels followed by analyses using diffusion tensor imaging and immunostaining for neuronal and glial markers. The results demonstrated that the P2 hydrogel considerably increased the number of axons and the distance of regeneration into the distal nerve stump. In summary, the method used to decellularize nerve tissue affects the efficacy of the resulting hydrogels to support regeneration after nerve injury.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- MRI
- biosynthetic conduit
- decellularized nerve tissue
- diffusion tensor imaging
- nerve-derived hydrogel
- peripheral nerve injury
Publication and Content Type
- ref (subject category)
- art (subject category)
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Kumar Kuna, Vija ...
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Lundgren, André
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Anerillas, Luis ...
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Kelk, Peyman
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Brohlin, Maria, ...
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Wiberg, Mikael
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Kingham, Paul J.
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Novikova, Ludmil ...
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Andersson, Gusta ...
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Novikov, Lev N.
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Umeå University