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Type IV Collagen in...
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Lindgren, MoaUmeå universitet,Kirurgi
(author)
Type IV Collagen in Human Colorectal Liver Metastases—Cellular Origin and a Circulating Biomarker
- Article/chapterEnglish2022
Publisher, publication year, extent ...
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2022-07-13
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MDPI,2022
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-199091
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-199091URI
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https://doi.org/10.3390/cancers14143396DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Circulating type IV collagen (cCOL IV) is a potential biomarker for patients with colorectal liver metastases (CLM) who present with elevated levels of COL IV in both CLM tissue and circulation. This study aimed to establish the cellular origin of elevated levels of COL IV and analyze circulating COL IV in CLM patients. The cellular source was established through in situ hybridization, immunohistochemical staining, and morphological evaluation. Cellular expression in vitro was assessed by immunofluorescence. Tissue expression of COL IV-degrading matrix metalloproteinases (MMPs)-2, -7, -9, and -13 was studied with immunohistochemical staining. Plasma levels of COL IV in CLM patients and healthy controls were analyzed with ELISA. This study shows that cancer-associated fibroblasts (CAFs) express COL IV in the stroma of CLM and that COL IV is expressed in vitro by fibroblasts but not by tumor cells. MMP-2, -7, -9, and -13 are expressed in CLM tissue, mainly by hepatocytes and immune cells, and circulating COL IV is significantly elevated in CLM patients compared with healthy controls. Our study shows that stromal cells, not tumor cells, produce COL IV in CLM, and that circulating COL IV is elevated in patients with CLM.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Rask, GunillaUmeå universitet,Patologi,Kirurgi(Swepub:umu)guarak02
(author)
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Jonsson, JosefinUmeå universitet,Kirurgi(Swepub:umu)jojo0205
(author)
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Berglund, Anette,1960-Umeå universitet,Kirurgi(Swepub:umu)anbe0011
(author)
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Lundin, Christina,1970-Umeå universitet,Kirurgi(Swepub:umu)chlu0008
(author)
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Jonsson, PärUmeå universitet,Kemiska institutionen(Swepub:umu)parjon95
(author)
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Ljuslinder, Ingrid,1968-Umeå universitet,Onkologi(Swepub:umu)inlj0001
(author)
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Nyström, Hanna,1980-Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Kirurgi(Swepub:umu)haanym01
(author)
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Umeå universitetKirurgi
(creator_code:org_t)
Related titles
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In:Cancers: MDPI14:142072-6694
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