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Regulation of PaRBO...
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Nickolov, KaloianDepartment of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; Department of Ecology and Genetics, University of Oulu, Oulu, Finland
(author)
Regulation of PaRBOH1-mediated ROS production in Norway spruce by Ca2+ binding and phosphorylation
- Article/chapterEnglish2022
Publisher, publication year, extent ...
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2022-10-13
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Frontiers Media S.A.2022
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-200887
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-200887URI
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https://doi.org/10.3389/fpls.2022.978586DOI
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https://res.slu.se/id/publ/119942URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Plant respiratory burst oxidase homologs (RBOHs) are plasma membrane-localized NADPH oxidases that generate superoxide anion radicals, which then dismutate to H2O2, into the apoplast using cytoplasmic NADPH as an electron donor. PaRBOH1 is the most highly expressed RBOH gene in developing xylem as well as in a lignin-forming cell culture of Norway spruce (Picea abies L. Karst.). Since no previous information about regulation of gymnosperm RBOHs exist, our aim was to resolve how PaRBOH1 is regulated with a focus on phosphorylation. The N-terminal part of PaRBOH1 was found to contain several putative phosphorylation sites and a four-times repeated motif with similarities to the Botrytis-induced kinase 1 target site in Arabidopsis AtRBOHD. Phosphorylation was indicated for six of the sites in in vitro kinase assays using 15 amino-acid-long peptides for each of the predicted phosphotarget site in the presence of protein extracts of developing xylem. Serine and threonine residues showing positive response in the peptide assays were individually mutated to alanine (kinase-inactive) or to aspartate (phosphomimic), and the wild type PaRBOH1 and the mutated constructs transfected to human kidney embryogenic (HEK293T) cells with a low endogenous level of extracellular ROS production. ROS-producing assays with HEK cells showed that Ca2+ and phosphorylation synergistically activate the enzyme and identified several serine and threonine residues that are likely to be phosphorylated including a novel phosphorylation site not characterized in other plant species. These were further investigated with a phosphoproteomic study. Results of Norway spruce, the first gymnosperm species studied in relation to RBOH regulation, show that regulation of RBOH activity is conserved among seed plants.
Subject headings and genre
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Gauthier, AdrienDepartment of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; UniLaSalle, Agro-Ecology, Hydrogeochemistry, Environments & Resources, UP 2018.C101 of the Ministry in Charge of Agriculture (AGHYLE) Research Unit CS UP 2018.C101, Mont-Saint-Aignan, France
(author)
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Hashimoto, KenjiDepartment of Applied Biological Science, Tokyo University of Science, Noda, Japan
(author)
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Laitinen, TeresaDepartment of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland
(author)
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Väisänen, EnniDepartment of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland
(author)
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Paasela, TanjaDepartment of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; Natural Resources Institute Finland (Luke), Production Systems, Helsinki, Finland
(author)
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Soliymani, RabahMeilahti Clinical Proteomics Core Facility, Biochemistry & Dev. Biology, University of Helsinki, Biomedicum-Helsinki, Helsinki, Finland
(author)
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Kurusu, TakamitsuDepartment of Applied Biological Science, Tokyo University of Science, Noda, Japan; Department of Mechanical and Electrical Engineering, Suwa University of Science, Chino, Japan
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Himanen, KristiinaDepartment of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland
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Blokhina, OlgaOrganismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland
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Fagerstedt, Kurt V.Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland
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Jokipii-Lukkari, SoileUmeå universitet,Institutionen för fysiologisk botanik,Department of Ecology and Genetics, University of Oulu, Oulu, Finland
(author)
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Tuominen, HanneleSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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Häggman, HelyDepartment of Ecology and Genetics, University of Oulu, Oulu, Finland
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Wingsle, GunnarSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology(Swepub:slu)48677
(author)
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Teeri, Teemu H.Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland
(author)
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Kuchitsu, KazuyukiDepartment of Applied Biological Science, Tokyo University of Science, Noda, Japan
(author)
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Kärkönen, AnnaDepartment of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; Natural Resources Institute Finland (Luke), Production Systems, Helsinki, Finland
(author)
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Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; Department of Ecology and Genetics, University of Oulu, Oulu, FinlandDepartment of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; UniLaSalle, Agro-Ecology, Hydrogeochemistry, Environments & Resources, UP 2018.C101 of the Ministry in Charge of Agriculture (AGHYLE) Research Unit CS UP 2018.C101, Mont-Saint-Aignan, France
(creator_code:org_t)
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Sveriges lantbruksuniversitet
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In:Frontiers in Plant Science: Frontiers Media S.A.131664-462X
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Nickolov, Kaloia ...
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Gauthier, Adrien
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Hashimoto, Kenji
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Laitinen, Teresa
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Väisänen, Enni
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Paasela, Tanja
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Soliymani, Rabah
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Kurusu, Takamits ...
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Blokhina, Olga
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Fagerstedt, Kurt ...
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Jokipii-Lukkari, ...
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Tuominen, Hannel ...
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Häggman, Hely
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Wingsle, Gunnar
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Teeri, Teemu H.
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Kärkönen, Anna
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