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Transcriptome analy...
Transcriptome analysis reveals modulation of human stem cells from the Apical Papilla by species associated with dental root canal infection
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- Razghonova, Yelyzaveta (author)
- Department of Microbiology, Virology and Biotechnology, Mechnikov National University, Odesa, Ukraine
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- Zymovets, Valeriia (author)
- Umeå University,Umeå universitet,Institutionen för odontologi
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- Wadelius, Philip (author)
- Department of Endodontics, Region of Västerbotten, Umeå, Sweden
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- Rakhimova, Olena (author)
- Umeå University,Umeå universitet,Institutionen för odontologi
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- Manoharan, Lokeshwaran (author)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,EPI@LUND,Forskargrupper vid Lunds universitet,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine,Lund University Research Groups
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- Brundin, Malin (author)
- Umeå University,Umeå universitet,Institutionen för odontologi
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- Kelk, Peyman (author)
- Umeå University,Umeå universitet,Anatomi
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- Romani Vestman, Nelly (author)
- Umeå University,Umeå universitet,Institutionen för odontologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM)
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(creator_code:org_t)
- 2022-11-20
- 2022
- English.
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In: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 23:22
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Abstract
Subject headings
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- Interaction of oral bacteria with stem cells from the apical papilla (SCAP) can negatively affect the success of regenerative endodontic treatment (RET). Through RNA-seq transcriptomic analysis, we studied the effect of the oral bacteria Fusobacterium nucleatum and Enterococcus faecalis, as well as their supernatants enriched by bacterial metabolites, on the osteo- and dentinogenic potential of SCAPs in vitro. We performed bulk RNA-seq, on the basis of which differential expression analysis (DEG) and gene ontology enrichment analysis (GO) were performed. DEG analysis showed that E. faecalis supernatant had the greatest effect on SCAPs, whereas F. nucleatum supernatant had the least effect (Tanimoto coefficient = 0.05). GO term enrichment analysis indicated that F. nucleatum upregulates the immune and inflammatory response of SCAPs, and E. faecalis suppresses cell proliferation and cell division processes. SCAP transcriptome profiles showed that under the influence of E. faecalis the upregulation of VEGFA, Runx2, and TBX3 genes occurred, which may negatively affect the SCAP’s osteo- and odontogenic differentiation. F. nucleatum downregulates the expression of WDR5 and TBX2 and upregulates the expression of TBX3 and NFIL3 in SCAPs, the upregulation of which may be detrimental for SCAPs’ differentiation potential. In conclusion, the present study shows that in vitro, F. nucleatum, E. faecalis, and their metabolites are capable of up- or downregulating the expression of genes that are necessary for dentinogenic and osteogenic processes to varying degrees, which eventually may result in unsuccessful RET outcomes. Transposition to the clinical context merits some reservations, which should be approached with caution.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- dentinogenesis
- differential gene expression analysis (DEG)
- Enterococcus faecalis
- Fusobacterium nucleatum
- osteogenesis
- regenerative endodontic treatment (RET)
- stem cells from the apical papilla (SCAP)
- transcriptome analysis
- odontologi
- Odontology
Publication and Content Type
- ref (subject category)
- art (subject category)
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