Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: id:"swepub:oai:DiVA.org:umu-201627" > Cultivation Methods...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Berthold, AnneDepartment of Biology, Mt. Allison University, Canada (author)

Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia

Article/chapterEnglish2022

Publisher, publication year, extent ...

2022-11-25
MyJove Corporation,2022
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:umu-201627
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-201627URI
https://doi.org/10.3791/64431DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

The Borrelia consists of three groups of species, those of the Lyme borreliosis (LB) group, also known as B. burgdorferi sensu lato (s.l.) and recently reclassified into Borreliella, the relapsing fever (RF) group Borrelia, and a third reptile-associated group of spirochetes. Culture-based methods remain the gold standard for the laboratory detection of bacterial infections for both research and clinical work, as the culture of pathogens from bodily fluids or tissues directly detects replicating pathogens and provides source material for research. Borrelia and Borreliella spirochetes are fastidious and slow growing, and thus are not commonly cultured for clinical purposes; however, culture is necessary for research. This protocol demonstrates the methodology and recipes required to successfully culture LB and RF spirochetes, including all recognized species from B. burgdorferi s.l. complex including B. afzelii, B. americana, B. andersonii, B. bavariensis, B. bissettii/bissettiae, B. burgdorferi sensu stricto (s.s.), B. californiensis, B. carolinensis, B. chilensis, B. finlandensis, B. garinii, B. japonica, B. kurtenbachii, B. lanei, B. lusitaniae, B. maritima, B. mayonii, B. spielmanii, B. tanukii, B. turdi, B. sinica, B. valaisiana, B. yangtzensis, and RFspirochetes, B. anserina, B. coriaceae, B. crocidurae, B. duttonii, B. hermsii, B. hispanica, B. persica, B. recurrentis, and B. miyamotoi. The basic medium for growing LB and RF spirochetes is the Barbour-Stoenner-Kelly (BSK-II or BSK-H) medium, which reliably supports the growth of spirochetes in established cultures. To be able to grow newly isolated Borrelia isolates from tick-or host-derived samples where the initial spirochete number is low in the inoculum, modified Kelly-Pettenkofer (MKP) medium is preferred. This medium also supports the growth of B. miyamotoi. The success of the cultivation of RF spirochetes also depends critically on the quality of ingredients.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

Faucillion, Marie-Line,1989-Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)(Swepub:umu)mafa0102 (author)
Nilsson, IngelaUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)(Swepub:umu)inni0005 (author)
Golovchenko, MarynaBiology Centre Czech Academy of Sciences, Institute of Parasitology, Czech Republic (author)
Lloyd, VettDepartment of Biology, Mt. Allison University, Canada (author)
Bergström, SvenUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)(Swepub:umu)svbe0001 (author)
Rudenko, NatalieBiology Centre Czech Academy of Sciences, Institute of Parasitology, Czech Republic (author)
Department of Biology, Mt. Allison University, CanadaInstitutionen för molekylärbiologi (Medicinska fakulteten) (creator_code:org_t)

Related titles

In:Journal of Visualized Experiments: MyJove Corporation2022:1891940-087X

Internet link

Find in a library

Journal of Visualized Experiments (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Berthold, Anne; Faucillion, Mari ...; Nilsson, Ingela; Golovchenko, Mar ...; Lloyd, Vett; Bergström, Sven; show more...; Rudenko, Natalie; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

Articles in the publication: Journal of Visua ...

By the university: Umeå University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se