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  • Gineste, CharlotteDepartment of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Illkirch, France (författare)

Enzymatically dissociated muscle fibers display rapid dedifferentiation and impaired mitochondrial calcium control

  • Artikel/kapitelEngelska2022

Förlag, utgivningsår, omfång ...

  • Elsevier,2022
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:umu-201747
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-201747URI
  • https://doi.org/10.1016/j.isci.2022.105654DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-104631URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:151792474URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Funding agencies:Swedish Research Council for Sport Science P2019-0060Association Francaise contre les Myopathies 16798German Research Foundation (DFG) FR2993/13-1United States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS)1F32AR057619 
  • Cells rapidly lose their physiological phenotype upon disruption of their extracellular matrix (ECM)-intracellular cytoskeleton interactions. By comparing adult mouse skeletal muscle fibers, isolated either by mechanical dissection or by collagenase-induced ECM digestion, we investigated acute effects of ECM disruption on cellular and mitochondrial morphology, transcriptomic signatures, and Ca2+ handling. RNA-sequencing showed striking differences in gene expression patterns between the two isolation methods with enzymatically dissociated fibers resembling myopathic phenotypes. Mitochondrial appearance was grossly similar in the two groups, but 3D electron microscopy revealed shorter and less branched mitochondria following enzymatic dissociation. Repeated contractions resulted in a prolonged mitochondrial Ca2+ accumulation in enzymatically dissociated fibers, which was partially prevented by cyclophilin inhibitors. Of importance, muscle fibers of mice with severe mitochondrial myopathy show pathognomonic mitochondrial Ca2+ accumulation during repeated contractions and this accumulation was concealed with enzymatic dissociation, making this an ambiguous method in studies of native intracellular Ca2+ fluxes.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Youhanna, SoniaDepartment of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden (författare)
  • Vorrink, Sabine U.Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden (författare)
  • Henriksson, SaraUmeå universitet,Kemiska institutionen,Umeå Core Facility for Electron Microscopy, Department of Chemistry, Umeå University, Umeå, Sweden(Swepub:umu)saaern00 (författare)
  • Hernández, AndrésDepartment of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; University of California Merced, Merced CA, USA (författare)
  • Cheng, Arthur J.Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; School of Kinesiology and Health Sciences, York University, Toronto, Canada (författare)
  • Chaillou, Thomas,1985-Örebro universitet,Institutionen för hälsovetenskaper,Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden(Swepub:oru)tcu (författare)
  • Buttgereit, AndreasInstitute of Medical Biotechnology, Department of Chemical and Biological Engineering, Friedrich- Alexander University of Erlangen-Nürnberg, Erlangen, Germany (författare)
  • Schneidereit, DominikInstitute of Medical Biotechnology, Department of Chemical and Biological Engineering, Friedrich- Alexander University of Erlangen-Nürnberg, Erlangen, Germany (författare)
  • Friedrich, OliverInstitute of Medical Biotechnology, Department of Chemical and Biological Engineering, Friedrich- Alexander University of Erlangen-Nürnberg, Erlangen, Germany (författare)
  • Hultenby, KjellKarolinska Institutet (författare)
  • Bruton, Joseph D.Karolinska Institutet (författare)
  • Ivarsson, NiklasDepartment of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden (författare)
  • Sandblad, LindaUmeå universitet,Kemiska institutionen,Umeå Core Facility for Electron Microscopy, Department of Chemistry, Umeå University, Umeå, Sweden(Swepub:umu)lisa0088 (författare)
  • Lauschke, Volker M.Karolinska Institutet (författare)
  • Westerblad, HåkanKarolinska Institutet (författare)
  • Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Illkirch, FranceDepartment of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:iScience: Elsevier25:122589-0042

Internetlänk

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  • iScience (Sök värdpublikationen i LIBRIS)

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