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Simultaneous membrane and RNA binding by tick-borne encephalitis virus capsid protein
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- Pulkkinen, Lauri Ilmari Aurelius (author)
- Faculty of Biological and Environmental Sciences, Molecular and Integrative Bioscience Research Programme, University of Helsinki, Helsinki, Finland; University of Helsinki, Helsinki Institute of Life Sciences-Institute of Biotechnology, Helsinki, Finland
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- Barrass, Sarah Victoria (author)
- Faculty of Biological and Environmental Sciences, Molecular and Integrative Bioscience Research Programme, University of Helsinki, Helsinki, Finland; University of Helsinki, Helsinki Institute of Life Sciences-Institute of Biotechnology, Helsinki, Finland
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- Lindgren, Marie (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Molekylär Infektionsmedicin, Sverige (MIMS)
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- 2023-02-14
- 2023
- English.
- In: PLoS Pathogens. - : Public Library of Science. - 1553-7366 .- 1553-7374. ; 19:2
- Journal article (peer-reviewed)
Abstract
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- Tick-borne encephalitis virus is an enveloped, pathogenic, RNA virus in the family Flaviviridae, genus Flavivirus. Viral particles are formed when the nucleocapsid, consisting of an RNA genome and multiple copies of the capsid protein, buds through the endoplasmic reticulum membrane and acquires the viral envelope and the associated proteins. The coordination of the nucleocapsid components to the sites of assembly and budding are poorly understood. Here, we investigate the interactions of the wild-type and truncated capsid proteins with membranes with biophysical methods and model membrane systems. We show that capsid protein initially binds membranes via electrostatic interactions with negatively-charged lipids, which is followed by membrane insertion. Additionally, we show that membrane-bound capsid protein can recruit viral genomic RNA. We confirm the biological relevance of the biophysical findings by using mass spectrometry to show that purified virions contain negatively-charged lipids. Our results suggest that nucleocapsid assembly is coordinated by negatively-charged membrane patches on the endoplasmic reticulum and that the capsid protein mediates direct contacts between the nucleocapsid and the membrane.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
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