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Detection of antinu...
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Bonroy, CarolienDepartment of Diagnostic Sciences, Ghent University, Ghent, Belgium; Department of Laboratory Medicine, University Hospital Ghent, Ghent, Belgium
(author)
Detection of antinuclear antibodies : recommendations from EFLM, EASI and ICAP
- Article/chapterEnglish2023
Publisher, publication year, extent ...
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2023-03-29
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Walter de Gruyter,2023
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-206769
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-206769URI
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https://doi.org/10.1515/cclm-2023-0209DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Objectives: Antinuclear antibodies (ANA) are important for the diagnosis of various autoimmune diseases. ANA are usually detected by indirect immunofluorescence assay (IFA) using HEp-2 cells (HEp-2 IFA). There are many variables influencing HEp-2 IFA results, such as subjective visual reading, serum screening dilution, substrate manufacturing, microscope components and conjugate. Newer developments on ANA testing that offer novel features adopted by some clinical laboratories include automated computer-assisted diagnosis (CAD) systems and solid phase assays (SPA).Methods: A group of experts reviewed current literature and established recommendations on methodological aspects of ANA testing. This process was supported by a two round Delphi exercise. International expert groups that participated in this initiative included (i) the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group “Autoimmunity Testing”; (ii) the European Autoimmune Standardization Initiative (EASI); and (iii) the International Consensus on ANA Patterns (ICAP).Results: In total, 35 recommendations/statements related to (i) ANA testing and reporting by HEp-2 IFA; (ii) HEp-2 IFA methodological aspects including substrate/conjugate selection and the application of CAD systems; (iii) quality assurance; (iv) HEp-2 IFA validation/verification approaches and (v) SPA were formulated. Globally, 95% of all submitted scores in the final Delphi round were above 6 (moderately agree, agree or strongly agree) and 85% above 7 (agree and strongly agree), indicating strong international support for the proposed recommendations.Conclusions: These recommendations are an important step to achieve high quality ANA testing.
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Vercammen, MartineDepartment of Laboratory Medicine, AZ Sint-Jan, Brugge, Belgium; Research Group REIM, Vrije Universiteit Brussel, Brussels, Belgium
(author)
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Fierz, WalterSchweizerischer Verband der Diagnostikindustrie (SVDI-ASID), Bern, Switzerland
(author)
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Andrade, Luis E.C.Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil; Immunology Division, Fleury Medicine and Health Laboratories, Sao Paulo, Brazil
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Van Hoovels, LieveDepartment of Laboratory Medicine, OLV Hospital, Aalst, Belgium; Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium
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Infantino, MariaImmunology and Allergology Laboratory, S. Giovanni di Dio Hospital, Florence, Italy
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Fritzler, Marvin J.Department of Medicine, Cumming School of Medicine, University of Calgary, AB, Calgary, Canada
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Bogdanos, DimitriosDepartment of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, University General Hospital of Larissa, Larissa, Greece
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Kozmar, AnaDepartment of Laboratory Diagnostics, University Hospital Centre Zagreb, Zagreb, Croatia
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Nespola, BenoitLaboratory of Immunology, Hôpitaux Universitaires de Strasbourg, Strasbourg, France
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Broeders, SylviaQuality of Laboratories, Sciensano, Ixelles, Belgium
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Patel, DinaUK NEQAS Immunology, Immunochemistry and Allergy, Sheffield Teaching Hospitals, Sheffield, United Kingdom
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Herold, ManfredDepartment of Internal Medicine II, Rheumatology Laboratory, Medical University of Innsbruck, Innsbruck, Austria
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Zheng, BingDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
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Chan, Eric Y.T.Department of Pathology, Queen Mary Hospital, Hong Kong, Hong Kong
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Uibo, RaivoDepartment of Immunology, Medical Faculty, University of Tartu, Tartu, Estonia
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Haapala, Anna-MaijaDepartment of Immunology, Fimlab Laboratories, Tampere, Finland
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Musset, LucileDepartment of Immunology, Assistance Publique-Hôpitaux de Paris, Groupe Hospitalier Pitié-Salpêtrière, Paris, France
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Sack, UlrichMedical Faculty, Leipzig University, Leipzig, Germany
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Nagy, GaborDepartment of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
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Sundic, TatjanaDepartment of Immunology and Transfusion Medicine, Haugesund Hospital, Helse Fonna, Haugesund, Norway
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Fischer, KatarzynaIndividual Laboratory for Rheumatologic Diagnostics, Pomeranian Medical University in Szczecin, Szczecin, Poland
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Rego De Sousa, Maria-JoséImmunopathology and Autoimmunity Department, Centro de Medicina Laboratorial Germano de Sousa, Lisbon, Portugal
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Vargas, Maria LuisaImmunology, Hospital Universitario de Badajoz, Badajoz, Spain
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Eriksson, Catharina,1955-Umeå universitet,Institutionen för klinisk mikrobiologi(Swepub:umu)caaern74
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Heijnen, IngmarImmunology, Laboratory Medicine, University Hospital Basel, Basel, Switzerland
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García-De La Torre, IgnacioDepartment of Immunology and Rheumatology, Hospital General de Occidente, Universidad de Guadalajara, Guadalajara, Mexico
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Carballo, Orlando GabrielLaboratory of Immunology, Hospital Carlos G. Durand, Buenos Aires, Argentina; Department of Microbiology and Immunology, Instituto Universitario, Hospital Italiano de Buenos Aires, Buenos Aires, Argentina
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Satoh, MinoruDepartment of Human, Information and Life Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan; Department of Medicine, Kitakyushu Yahata-Higashi Hospital, Kitakyushu, Japan
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Kim, Kyeong-HeeDepartment of Laboratory Medicine, Dong-A University College of Medicine, Busan, South Korea
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Chan, Edward K.L.Department of Oral Biology, University of Florida, FL, Gainesville, United States
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Damoiseaux, JanCentral Diagnostic Laboratory, Maastricht University Medical Center, Maastricht, Netherlands
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Lopez-Hoyos, MarcosImmunology Service, University Hospital Marques de Valdecilla-IDIVAL, University of Cantabria, Santander, Spain
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Bossuyt, XavierDepartment of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium; Department of Laboratory Medicine, University Hospital Leuven, Leuven, Belgium
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Department of Diagnostic Sciences, Ghent University, Ghent, Belgium; Department of Laboratory Medicine, University Hospital Ghent, Ghent, BelgiumDepartment of Laboratory Medicine, AZ Sint-Jan, Brugge, Belgium; Research Group REIM, Vrije Universiteit Brussel, Brussels, Belgium
(creator_code:org_t)
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In:Clinical Chemistry and Laboratory Medicine: Walter de Gruyter61:7, s. 1167-11981434-66211437-4331
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Bonroy, Carolien
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Vercammen, Marti ...
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Fierz, Walter
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Andrade, Luis E. ...
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Van Hoovels, Lie ...
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Infantino, Maria
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Fritzler, Marvin ...
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Bogdanos, Dimitr ...
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Kozmar, Ana
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Nespola, Benoit
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Broeders, Sylvia
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Patel, Dina
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Herold, Manfred
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Zheng, Bing
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Chan, Eric Y.T.
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Uibo, Raivo
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Haapala, Anna-Ma ...
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Musset, Lucile
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Sack, Ulrich
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Nagy, Gabor
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Sundic, Tatjana
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Fischer, Katarzy ...
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Rego De Sousa, M ...
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Vargas, Maria Lu ...
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Eriksson, Cathar ...
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Heijnen, Ingmar
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García-De La Tor ...
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Carballo, Orland ...
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Satoh, Minoru
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Kim, Kyeong-Hee
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Chan, Edward K.L ...
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Damoiseaux, Jan
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Lopez-Hoyos, Mar ...
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Bossuyt, Xavier
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