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Functional signific...
Functional significance of asymmetrical retention of parental alleles in a hybrid pine species complex
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- Qu, Chang (författare)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry and Northeast Forestry University, Beijing, China
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- Kao, Hong-Na (författare)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry and Northeast Forestry University, Beijing, China
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- Xu, Hui (författare)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry and Northeast Forestry University, Beijing, China
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- Wang, Bao-Sheng (författare)
- Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
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- Yang, Zhi-Ling (författare)
- Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Yunnan, Menglun, China
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- Yang, Qi (författare)
- State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Zhejiang, Hangzhou, China
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- Liu, Gui-Feng (författare)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry and Northeast Forestry University, Beijing, China
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- Wang, Xiao-Ru (författare)
- Umeå universitet,Institutionen för ekologi, miljö och geovetenskap,Umeå Plant Science Centre (UPSC)
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- Liu, Yan-Jing (författare)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry and Northeast Forestry University, Beijing, China
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- Zeng, Qing-Yin (författare)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry and Northeast Forestry University, Beijing, China
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(creator_code:org_t)
- 2023-03-10
- 2024
- Engelska.
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Ingår i: Journal of Systematics and Evolution. - : John Wiley & Sons. - 1674-4918 .- 1759-6831. ; 62:1, s. 135-148
- Relaterad länk:
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Hybrid genomes usually harbor asymmetrical parental contributions. However, it is challenging to infer the functional significance of asymmetrical retention of parental alleles in hybrid populations of conifer trees. Here we investigated the diversity in the glutathione S-transferase (GST) gene family in a hybrid pine Pinus densata and its parents (Pinus tabuliformis and Pinus yunnanensis). Plant GSTs play major roles in protecting plants against biotic and abiotic stresses. In this study, 19 orthologous groups of GST genes were identified and cloned from these three species. We examined their expression in different tissues, and then purified the corresponding proteins to characterize their enzymatic activities and specificities toward different substrates. We found that among the 19 GST orthologous groups, divergence in gene expression and in enzymatic activities toward different substrates was prevalent. P. densata preferentially retained P. yunnanensis-like GSTs for 17 out of the 19 gene loci. We determined the first GST crystal structure from conifer species at a resolution of 2.19 Å. Based on this structure, we performed site-directed mutagenesis to replace amino acid residuals in different wild-types of GSTs to understand their functional impacts. Reciprocal replacement of amino acid residuals in native GSTs of P. densata and P. tabuliformis demonstrated significant changes in enzyme functions and identified key sites controlling GSTs activities. This study illustrates an approach to evaluating the functional significance of sequence variations in conifer genomes. Our study also sheds light on plausible mechanisms for controlling the selective retention of parental alleles in the P. densata genome.
Ämnesord
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
Nyckelord
- enzymatic function
- functional divergence
- gene expression
- glutathione S-transferases
- homoploid hybrid species
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- art (ämneskategori)
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Qu, Chang
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Kao, Hong-Na
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Xu, Hui
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Wang, Bao-Sheng
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Yang, Zhi-Ling
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Yang, Qi
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visa fler...
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Liu, Gui-Feng
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Wang, Xiao-Ru
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Liu, Yan-Jing
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Zeng, Qing-Yin
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