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Material stiffness ...
Material stiffness in cooperation with macrophage paracrine signals determines the tenogenic differentiation of mesenchymal stem cells
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- Sheng, Renwang (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Liu, Jia (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Zhang, Wei (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, China; China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China
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- Luo, Yifan (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Chen, Zhixuan (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Chi, Jiayu (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Mo, Qingyun (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Wang, Mingyue (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Sun, Yuzhi (författare)
- Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China; Department of Orthopaedic Surgery, Institute of Digital Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
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- Liu, Chuanquan (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Zhang, Yanan (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Zhu, Yue (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Kuang, Baian (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Yan, Chunguang (författare)
- School of Medicine, Southeast University, Nanjing, China
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- Liu, Haoyang (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China
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- Backman, Ludvig J. (författare)
- Umeå universitet,Anatomi
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- Chen, Jialin (författare)
- School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, China; China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China
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(creator_code:org_t)
- John Wiley & Sons, 2023
- 2023
- Engelska.
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Ingår i: Advanced Science. - : John Wiley & Sons. - 2198-3844. ; 10:17
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Stiffness is an important physical property of biomaterials that determines stem cell fate. Guiding stem cell differentiation via stiffness modulation has been considered in tissue engineering. However, the mechanism by which material stiffness regulates stem cell differentiation into the tendon lineage remains controversial. Increasing evidence demonstrates that immune cells interact with implanted biomaterials and regulate stem cell behaviors via paracrine signaling; however, the role of this mechanism in tendon differentiation is not clear. In this study, polydimethylsiloxane (PDMS) substrates with different stiffnesses are developed, and the tenogenic differentiation of mesenchymal stem cells (MSCs) exposed to different stiffnesses and macrophage paracrine signals is investigated. The results reveal that lower stiffnesses facilitates tenogenic differentiation of MSCs, while macrophage paracrine signals at these stiffnesses suppress the differentiation. When exposed to these two stimuli, MSCs still exhibit enhanced tendon differentiation, which is further elucidated by global proteomic analysis. Following subcutaneous implantation in rats for 2 weeks, soft biomaterial induces only low inflammation and promotes tendon-like tissue formation. In conclusion, the study demonstrates that soft, rather than stiff, material has a greater potential to guide tenogenic differentiation of stem cells, which provides comprehensive evidence for optimized bioactive scaffold design in tendon tissue engineering.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- macrophage polarization
- proteomics
- stem cell
- stiffness
- tenogenic differentiation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Sheng, Renwang
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Liu, Jia
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Zhang, Wei
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Luo, Yifan
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Chen, Zhixuan
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Chi, Jiayu
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visa fler...
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Mo, Qingyun
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Wang, Mingyue
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Sun, Yuzhi
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Liu, Chuanquan
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Zhang, Yanan
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Zhu, Yue
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Kuang, Baian
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Yan, Chunguang
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Liu, Haoyang
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Backman, Ludvig ...
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Chen, Jialin
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Advanced Science
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Umeå universitet