Fucose binding motifs on mucin core glycopeptides impact bacterial lectin recognition

Behren, SandraUmeå universitet,Kemiska institutionen,Leibniz-Institut für Analytische Wissenschaften, Dortmund, Germany (author)

Mucin glycoproteins are essential components of the mucosal barrier, which protects the host from pathogens. Throughout evolution, bacteria have developed strategies to modulate and penetrate this barrier, and cause virulence by interacting with mucin O-glycans at the epithelial cell-surface. O-fucosylated glycan epitopes on mucins are key ligands of many bacterial lectins. Here, a chemoenzymatic synthesis strategy is described to prepare a library of fucosylated mucin core glycopeptides to enable studies of mucin-interacting and fucose-binding bacterial lectins. Glycan cores with biologically important Lewis and H-antigens were prepared decorating the peptide backbone at different sites and densities. The fucosylated mucin glycopeptides were applied in microarray binding studies to explore the importance of glycan core and peptide backbone presentation of these antigens in binding interactions with the P. aeruginosa lectin LecB and the C. difficile toxin A.

Yu, JinLeibniz-Institut für Analytische Wissenschaften, Dortmund, Germany; Glycosciences Laboratory, Imperial College London, London, United Kingdom (author)
Pett, ChristianUmeå universitet,Kemiska institutionen,Leibniz-Institut für Analytische Wissenschaften, Dortmund, Germany(Swepub:umu)chpe0083 (author)
Schorlemer, ManuelUmeå universitet,Kemiska institutionen,Leibniz-Institut für Analytische Wissenschaften, Dortmund, Germany(Swepub:umu)masc0109 (author)
Heine, ViktoriaLaboratory for Biomaterials, Institute of Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany (author)
Fischöder, ThomasLaboratory for Biomaterials, Institute of Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany (author)
Elling, LotharLaboratory for Biomaterials, Institute of Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany (author)
Westerlind, UlrikaUmeå universitet,Kemiska institutionen,Leibniz-Institut für Analytische Wissenschaften, Dortmund, Germany(Swepub:umu)ulwe0024 (author)
Umeå universitetKemiska institutionen (creator_code:org_t)

By the author/editor: Behren, Sandra; Yu, Jin; Pett, Christian; Schorlemer, Manu ...; Heine, Viktoria; Fischöder, Thoma ...; show more...; Elling, Lothar; Westerlind, Ulri ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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