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A phloem-localized Arabidopsis metacaspase (AtMC3) improves drought tolerance

Pitsili, Eugenia (författare)
Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, Spain; Department of Plant Systems Biology, Department of Plant Biotechnology and Bioinformatics, Flanders Institute for Biotechnology, Ghent University, Ghent, Belgium
Rodriguez-Trevino, Ricardo (författare)
Group of Plant Vascular Development, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland
Ruiz-Solani, Nerea (författare)
Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, Spain
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Demir, Fatih (författare)
Central Institute for Engineering, Electronics and Analytics, ZEA-3, Forschungszentrum Jülich GmbH, Jülich, Germany; Cologne Excellence Cluster Cellular Stress Response in Aging-Associated Diseases (CECAD), Department of Chemistry, University of Cologne, Medical Faculty and University Hospital, Institute of Biochemistry, Joseph-Stelzmann-Str. 26, Cologne, Germany
Kastanaki, Elizabeth (författare)
Group of Plant Vascular Development, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland
Dambire, Charlene (författare)
School of Biosciences, University of Nottingham, Loughborough, United Kingdom
de Pedro-Jové, Roger (författare)
Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, Spain
Vercammen, Dominique (författare)
Department of Plant Systems Biology, Department of Plant Biotechnology and Bioinformatics, Flanders Institute for Biotechnology, Ghent University, Ghent, Belgium
Salguero-Linares, Jose (författare)
Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, Spain
Hall, Hardy (författare)
Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
Mantz, Melissa (författare)
Central Institute for Engineering, Electronics and Analytics, ZEA-3, Forschungszentrum Jülich GmbH, Jülich, Germany; Cologne Excellence Cluster Cellular Stress Response in Aging-Associated Diseases (CECAD), Department of Chemistry, University of Cologne, Medical Faculty and University Hospital, Institute of Biochemistry, Joseph-Stelzmann-Str. 26, Cologne, Germany
Schuler, Martin (författare)
Group of Plant Vascular Development, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland
Tuominen, Hannele, 1964- (författare)
Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
Van Breusegem, Frank (författare)
Department of Plant Systems Biology, Department of Plant Biotechnology and Bioinformatics, Flanders Institute for Biotechnology, Ghent University, Ghent, Belgium
Valls, Marc (författare)
Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, Spain; Department of Genetics, Universitat de Barcelona, Barcelona, Spain
Munné-Bosch, Sergi (författare)
Department of Evolutionary Biology, Ecology and Environmental Sciences, Faculty of Biology, Universitat de Barcelona, Avinguda Diagonal 643, Barcelona, Spain; Institute of Research in Biodiversity (IRBio-UB), Universitat de Barcelona, Barcelona, Spain
Holdsworth, Michael J. (författare)
School of Biosciences, University of Nottingham, Loughborough, United Kingdom
Huesgen, Pitter F. (författare)
Central Institute for Engineering, Electronics and Analytics, ZEA-3, Forschungszentrum Jülich GmbH, Jülich, Germany; Cologne Excellence Cluster Cellular Stress Response in Aging-Associated Diseases (CECAD), Department of Chemistry, University of Cologne, Medical Faculty and University Hospital, Institute of Biochemistry, Joseph-Stelzmann-Str. 26, Cologne, Germany
Rodriguez-Villalon, Antia (författare)
Group of Plant Vascular Development, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland
Coll, Nuria S. (författare)
Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, Spain; Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain
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 (creator_code:org_t)
John Wiley & Sons, 2023
2023
Engelska.
Ingår i: New Phytologist. - : John Wiley & Sons. - 0028-646X .- 1469-8137. ; 239, s. 1281-1299
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Increasing drought phenomena pose a serious threat to agricultural productivity. Although plants have multiple ways to respond to the complexity of drought stress, the underlying mechanisms of stress sensing and signaling remain unclear. The role of the vasculature, in particular the phloem, in facilitating inter-organ communication is critical and poorly understood. Combining genetic, proteomic and physiological approaches, we investigated the role of AtMC3, a phloem-specific member of the metacaspase family, in osmotic stress responses in Arabidopsis thaliana. Analyses of the proteome in plants with altered AtMC3 levels revealed differential abundance of proteins related to osmotic stress pointing into a role of the protein in water-stress-related responses. Overexpression of AtMC3 conferred drought tolerance by enhancing the differentiation of specific vascular tissues and maintaining higher levels of vascular-mediated transportation, while plants lacking the protein showed an impaired response to drought and inability to respond effectively to the hormone abscisic acid. Overall, our data highlight the importance of AtMC3 and vascular plasticity in fine-tuning early drought responses at the whole plant level without affecting growth or yield.

Ämnesord

NATURVETENSKAP  -- Biologi -- Botanik (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Botany (hsv//eng)

Nyckelord

abscisic acid
Arabidopsis thaliana
drought
hypoxia
metacaspases
osmotic stress
phloem

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