Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand

Dmowski, MichalInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, Warsaw, Poland (författare)

The generally accepted model assumes that leading strand synthesis is performed by Pol ε, while lagging-strand synthesis is catalyzed by Pol δ. Pol ε has been shown to target the leading strand by interacting with the CMG helicase [Cdc45 Mcm2–7 GINS(Psf1–3, Sld5)]. Proper functioning of the CMG-Pol ɛ, the helicase-polymerase complex is essential for its progression and the fidelity of DNA replication. Dpb2p, the essential non-catalytic subunit of Pol ε plays a key role in maintaining the correct architecture of the replisome by acting as a link between Pol ε and the CMG complex. Using a temperature-sensitive dpb2–100 mutant previously isolated in our laboratory, and a genetic system which takes advantage of a distinct mutational signature of the Pol δ-L612M variant which allows detection of the involvement of Pol δ in the replication of particular DNA strands we show that in yeast cells with an impaired Dpb2 subunit, the contribution of Pol δ to the replication of the leading strand is significantly increased.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Medicinsk genetik hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Medical Genetics hsv//eng
CMG (Cdc45 Mcm2–7 GINS)
DNA polymerase delta
DNA polymerase epsilon
DNA replication fidelity
Dpb2
Genome stability
Pol δ
Pol ε
Replication fork

Makiela-Dzbenska, KarolinaInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, Warsaw, Poland (författare)
Sharma, SushmaUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)sush0003 (författare)
Chabes, Andrei,ProfessorUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)anch0002 (författare)
Fijalkowska, Iwona J.Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, Warsaw, Poland (författare)
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, Warsaw, PolandInstitutionen för medicinsk kemi och biofysik (creator_code:org_t)

Av författaren/redakt...: Dmowski, Michal; Makiela-Dzbenska ...; Sharma, Sushma; Chabes, Andrei, ...; Fijalkowska, Iwo ...

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