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Detection of lymph ...
Detection of lymph node metastasis in colon cancer by ectopically expressed fibroblast markers FOXQ1 and THBS2
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- Ali, Haytham (author)
- Umeå University,Umeå universitet,Institutionen för klinisk mikrobiologi,Onkologi,Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Oman
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- AbdelMageed, Manar (author)
- Umeå University,Umeå universitet,Institutionen för klinisk mikrobiologi,Onkologi,Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
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- Ohlsson, Lina (author)
- Umeå University,Umeå universitet,Institutionen för klinisk mikrobiologi
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- Lindmark, Gudrun (author)
- Lund University,Lunds universitet,Kirurgi,Forskargrupper vid Lunds universitet,Surgery,Lund University Research Groups
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- Hammarström, Marie-Louise (author)
- Umeå University,Umeå universitet,Institutionen för klinisk mikrobiologi
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- Hammarström, Sten (author)
- Umeå University,Umeå universitet,Institutionen för klinisk mikrobiologi
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- Sitohy, Basel (author)
- Umeå University,Umeå universitet,Institutionen för klinisk mikrobiologi,Onkologi
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(creator_code:org_t)
- Frontiers Media S.A. 2023
- 2023
- English.
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In: Frontiers in Oncology. - : Frontiers Media S.A.. - 2234-943X. ; 13
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Abstract
Subject headings
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- Introduction: Approximately 25% of colon cancer (CC) patients having curative surgery will relapse. Therefore, it is crucial to identify patients with increased recurrence risk to offer them adjuvant chemotherapy. Three markers with prominent expression in fibroblasts: forkhead box Q1 (FOXQ1), matrix metalloproteinase-11 (MMP11), and thrombospondin-2 (THBS2), and the fibroblast expressed chemokine CXCL12 were selected for studies because of the critical role of fibroblasts in the microenvironment of the tumor.Methods: The expression levels of the biomarkers were assessed in primary CC tumors, lymph nodes of CC patients and controls, and CC cell lines at mRNA and protein levels by real-time qRT-PCR and immunohistochemistry, respectively.Results: FOXQ1, MMP11, and THBS2 mRNAs were expressed at significantly higher levels in primary tumors compared to normal colon (P=0.002, P<0.0001, and P<0.0001, respectively). In contrast, CXCL12 mRNA levels were higher in normal colon tissue. FOXQ1, MMP11, and THBS2 levels were also expressed at significantly higher levels in metastasis-positive lymph nodes compared to both metastasis-negative- and control nodes (P<0.0001/P=0.002, P<0.0001/P<0.0001, and P<0.0001/P<0.0001, respectively). Immuno-morphometry revealed that 30–40% of the tumor cells expressed FOXQ1, MMP11, and THBS2. FOXQ1 and THBS2 were barely detected in normal colon epithelium (P<0.0001), while MMP11 was expressed in normal colon epithelium at high levels.Discussion: We conclude that CC tumor cells show ectopic expression of FOXQ1 and THBS2 possibly making these tumor cells independent of fibroblast cell support. The high expression levels of these two biomarkers in metastatic lymph nodes suggest that they are potential indicators of patients at risk for recurrence.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- colon cancer
- CXCL12
- fibroblasts
- FOXQ1
- immunohistochemistry
- MMP11
- qRT-PCR
- THBS2
- colon cancer
- CXCL12
- fibroblasts
- FOXQ1
- immunohistochemistry
- MMP11
- qRT-PCR
- THBS2
Publication and Content Type
- ref (subject category)
- art (subject category)
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