WFRF:(Li Wen Jun)
Sökning: WFRF:(Li Wen Jun) > (2007-2009) > MEK1/2 regulates mi...
LIBRIS Formathandbok (Information om MARC21)
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02892naa a2200373 4500 | |
| 001 | oai:DiVA.org:umu-22394 | |
| 003 | SwePub | |
| 008 | 090507s2007 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-223942 URI |
| 040 | a (SwePub)umu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Yu, Ling-Zhu4 aut |
| 245 | 1 0 | a MEK1/2 regulates microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte meiotic maturation. |
| 264 | 1 | c 2007 |
| 338 | a print2 rdacarrier | |
| 520 | a It is well known that MAPK plays pivotal roles in oocyte maturation, but the function of MEK (MAPK kinase) remains unknown. We have studied the expression, subcellular localization and functional roles of MEK during meiotic maturation of mouse oocytes. Firstly, we found that MEK1/2 phoshorylation (p-MEK1/2, indicative of MEK activation) was low in GV (germinal vesicle) stage, increased 2h after GVBD (germinal vesicle breakdown), and reached the maximum at metaphase II. Secondly, we found that P-MEK1/2 was restricted in the GV prior to GVBD. In prometaphase I and metaphase I, P-MEK1/2 was mainly associated with the spindle, especially with the spindle poles. At anaphase I and telophase I, p-MEK1/2 became diffusely distributed in the region between the separating chromosomes, and then became associated with the midbody. The association of p-MEK1/2 with spindle poles was further confirmed by its colocalization with the centrosomal proteins, gamma-tubulin and NuMA. Thirdly, we have investigated the possible functional role of MEK1/2 activation by intravenous administration and intrabursal injection of a specific MEK inhibitor, U0126, and by microinjection of MEK siRNA into oocytes. All these manipulations cause disorganized spindle poles and spindle structure, misaligned chromosomes and larger than normal polar bodies. Our results suggest that MEK1/2 may function as a centrosomal protein and may have roles in microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte maturation. | |
| 700 | 1 | a Xiong, Bo4 aut |
| 700 | 1 | a Gao, Wen-Xue4 aut |
| 700 | 1 | a Wang, Chun-Min4 aut |
| 700 | 1 | a Zhong, Zhi-Sheng4 aut |
| 700 | 1 | a Huo, Li-Jun4 aut |
| 700 | 1 | a Wang, Qiang4 aut |
| 700 | 1 | a Hou, Yi4 aut |
| 700 | 1 | a Liu, Kuiu Umeå universitet,Institutionen för medicinsk kemi och biofysik4 aut0 (Swepub:umu)kuli0001 |
| 700 | 1 | a Liu, X Johné4 aut |
| 700 | 1 | a Schatten, Heide4 aut |
| 700 | 1 | a Chen, Da-Yuan4 aut |
| 700 | 1 | a Sun, Qing-Yuan4 aut |
| 710 | 2 | a Umeå universitetb Institutionen för medicinsk kemi och biofysik4 org |
| 773 | 0 | t Cell cycle (Georgetown, Tex.)g 6:3, s. 330-8q 6:3<330-8x 1551-4005 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-22394 |
Hitta via bibliotek
- Cell cycle (Georgetown, Tex.) (Sök värdpublikationen i LIBRIS)
Till lärosätets databas
Hitta mer i SwePub
- Av författaren/redakt...
- Yu, Ling-Zhu
- Xiong, Bo
- Gao, Wen-Xue
- Wang, Chun-Min
- Zhong, Zhi-Sheng
- Huo, Li-Jun
- visa fler...
- Wang, Qiang
- Hou, Yi
- Liu, Kui
- Liu, X Johné
- Schatten, Heide
- Chen, Da-Yuan
- Sun, Qing-Yuan
- visa färre...
- Artiklar i publikationen
- Cell cycle (Geor ...
- Av lärosätet
- Umeå universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
