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On-line mass spectr...
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Beckmann, KatrinSchool of Biology, Australian National University, Canberra, ACT 0200 Australia,Max Planck Institut für Bioanorganische Chemie, 45470 Mülheim an der Ruhr, Germany,Australian Natl Univ, Sch Biol, Canberra, ACT 0200, Australia.;Max Planck Inst Bioanorgan Chem, D-45470 Mulheim, Germany.
(author)
On-line mass spectrometry : membrane inlet sampling
- Article/chapterEnglish2009
Publisher, publication year, extent ...
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2009-08-04
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Springer Netherlands,2009
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-25437
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-25437URI
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https://doi.org/10.1007/s11120-009-9474-7DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-514134URI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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SpringerLink Date Monday, August 03, 2009
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Significant insights into plant photosynthesis and respiration have been achieved using membrane inlet mass spectrometry (MIMS) for the analysis of stable isotope distribution of gases. The MIMS approach is based on using a gas permeable membrane to enable the entry of gas molecules into the mass spectrometer source. This is a simple yet durable approach for the analysis of volatile gases, particularly atmospheric gases. The MIMS technique strongly lends itself to the study of reaction flux where isotopic labeling is employed to differentiate two competing processes; i.e., O2 evolution versus O2 uptake reactions from PSII or terminal oxidase/rubisco reactions. Such investigations have been used for in vitro studies of whole leaves and isolated cells. The MIMS approach is also able to follow rates of isotopic exchange, which is useful for obtaining chemical exchange rates. These types of measurements have been employed for oxygen ligand exchange in PSII and to discern reaction rates of the carbonic anhydrase reactions. Recent developments have also engaged MIMS for online isotopic fractionation and for the study of reactions in inorganic systems that are capable of water splitting or H2 generation. The simplicity of the sampling approach coupled to the high sensitivity of modern instrumentation is a reason for the growing applicability of this technique for a range of problems in plant photosynthesis and respiration. This review offers some insights into the sampling approaches and the experiments that have been conducted with MIMS.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Messinger, JohannesUmeå universitet,Kemiska institutionen,School of Biology, Australian National University, Canberra, ACT 0200 Australia,Australian Natl Univ, Sch Biol, Canberra, ACT 0200, Australia.;Umea Univ, Dept Chem, S-90187 Umea, Sweden.(Swepub:uu)johme269
(author)
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Badger, Murray RonaldSchool of Biology, Australian National University, Canberra, ACT 0200 Australia,Australian Natl Univ, Sch Biol, Canberra, ACT 0200, Australia.
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Wydrzynski, TomSchool of Biology, Australian National University, Canberra, ACT 0200 Australia,Australian Natl Univ, Sch Biol, Canberra, ACT 0200, Australia.
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Hillier, WarwickSchool of Biology, Australian National University, Canberra, ACT 0200 Australia,Australian Natl Univ, Sch Biol, Canberra, ACT 0200, Australia.
(author)
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School of Biology, Australian National University, Canberra, ACT 0200 AustraliaMax Planck Institut für Bioanorganische Chemie, 45470 Mülheim an der Ruhr, Germany
(creator_code:org_t)
Related titles
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In:Photosynthesis Research: Springer Netherlands102:2-3, s. 511-5220166-85951573-5079
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