Vandetanib alters the protein pattern in malignant glioma and normal brain in the BT4C rat glioma model

The treatment of glioblastoma is unsatisfactory. Improved understanding of the biological effects of treatment, together with development of new tools to predict outcome of the initiated treatment are therefore of great need. Vandetanib (ZD6474) is mainly a vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) receptor tyrosine kinase inhibitor. This study investigated the pattern of protein expression in brain tumor and normal brain tissue, following treatment with vandetanib in a rat glioma model. BT4C-cells were stereotactically implanted into the brain of BD IX rats. The rats were divided into three different experiments. The treatment schedule for experiments one and two consisted of daily, oral doses of vandetanib from day 6 until day 12 or 20 after implantation, respectively. In the third experiment, each animal received a single dose of vandetanib on day 19 after implantation and was then sacrificed 2, 8 or 24 h thereafter. The protein expression profiles were analyzed by SELDI-TOF-MS and evaluated with multivariate statistical methods. Following treatment with vandetanib, we found significantly altered protein expression pattern in malignant glioma and normal brain. Analyzing protein spectra is an interesting option to assess biological effects induced in brain tissue by signal transduction inhibitors such as vandetanib.

Sandström, MariaUmeå universitet,Onkologi(Swepub:umu)maalin96 (författare)
Henriksson, RogerUmeå universitet,Onkologi(Swepub:umu)rohe0003 (författare)
Antti, HenrikUmeå universitet,Kemiska institutionen(Swepub:umu)hean0004 (författare)
Johansson, MikaelUmeå universitet,Onkologi(Swepub:umu)mijo0025 (författare)
Bergenheim, A TommyUmeå universitet,Neurokirurgi(Swepub:umu)tobe0004 (författare)
Umeå universitetOnkologi (creator_code:org_t)

Ingår i:International Journal of Cancer: Spandidos Publications37:4, s. 879-8900020-71361097-02151019-64391791-2423

Av författaren/redakt...: Wibom, Carl, 197 ...; Sandström, Maria; Henriksson, Roge ...; Antti, Henrik; Johansson, Mikae ...; Bergenheim, A To ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.