Force measuring optical tweezers system for long time measurements of P pili stability

A force-measuring optical tweezers instrumentation and long time measurements of the elongation and retraction of bacterial fimbriae from Uropathogenic E. coli (UPEC) under strain are presented. The instrumentation is presented in some detail. Special emphasis is given to measures taken to reduce the influence of noise and drifts in the system and from the surrounding, which makes long term force measurements possible. Individual P pili from UPEC bacteria were used as a biological model system for repetitive unfolding and refolding cycles of bacterial fimbriae under equilibrium conditions. P pili have evolved into a three-dimensional helix-like structure, the PapA rod, that can be successively and significantly elongated and/or unfolded when exposed to external forces. The instrumentation is used for characterization of the force-vs.-elongation response of the PapA rod of individual P pili, with emphasis on the long time stability of the forced unfolding and refolding of the helical structure of the PapA rod. The results show that the PapA rod is capable of withstanding extensive strain, leading to a complete unfolding of the helical structure, repetitive times during the life cycle of a bacterium without any noticeable alteration of the mechanical properties of the P pili. This function is believed to be importance for UPEC bacteria in vivo since it provides a close contact to a host cell (which is an initial step of invasion) despite urine cleaning attempts.

Fällman, ErikUmeå universitet,Institutionen för fysik(Swepub:umu)erfa0001 (författare)
Uhlin, Bernt EricUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Uhlin(Swepub:umu)beuh0001 (författare)
Axner, OveUmeå universitet,Institutionen för fysik(Swepub:umu)ovax0001 (författare)
Umeå universitetInstitutionen för fysik (creator_code:org_t)

Ingår i:Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV: SPIE, s. 608810-081946130X9780819461308

Av författaren/redakt...: Andersson, Magnu ...; Fällman, Erik; Uhlin, Bernt Eri ...; Axner, Ove

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