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Release of the type...
Release of the type I secreted α-haemolysin via outer membrane vesicles from Escherichia coli
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- Balsalobre, Carlos (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Silván, José Manuel (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Berglund, Stina (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bernt Eric Uhlin
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- Mizunoe, Yoshimitsu (författare)
- Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan
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- Uhlin, Bernt Eric (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Nyunt Wai, Sun (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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(creator_code:org_t)
- Hoboken, NJ, United States : Wiley-Blackwell, 2006
- 2006
- Engelska.
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Ingår i: Molecular Microbiology. - Hoboken, NJ, United States : Wiley-Blackwell. - 0950-382X .- 1365-2958. ; 59:1, s. 99-112
- Relaterad länk:
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https://onlinelibrar...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The α-haemolysin is an important virulence factor commonly expressed by extraintestinal pathogenic Escherichia coli. The secretion of the α-haemolysin is mediated by the type I secretion system and the toxin reaches the extracellular space without the formation of periplasmic intermediates presumably in a soluble form. Surprisingly, we found that a fraction of this type I secreted protein is located within outer membrane vesicles (OMVs) that are released by the bacteria. The α-haemolysin appeared very tightly associated with the OMVs as judged by dissociation assays and proteinase susceptibility tests. The α-haemolysin in OMVs was cytotoxically active and caused lysis of red blood cells. The OMVs containing the α-haemolysin were distinct from the OMVs not containing α-haemolysin, showing a lower density. Furthermore, they differed in protein composition and one component of the type I secretion system, the TolC protein, was found in the lower density vesicles. Studies of natural isolates of E. coli demonstrated that the localization of α-haemolysin in OMVs is a common feature among haemolytic strains. We propose an alternative pathway for the transport of the type I secreted α-haemolysin from the bacteria to the host cells during bacterial infections.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- Escherichia coli
- OMVs
- outer membrane vesicles
- haemolysin
- UPEC
- Medical microbiology
- Medicinsk mikrobiologi
- molekylärbiologi
- Molecular Biology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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