T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components.

The mechanisms controlling the formation of pseudopodia and other active cell edges in T lymphocytes are not understood. We show here that T lymphocytes express thrombospondin-1 (TSP-1). TSP-1 in T lymphocytes has a high turnover as shown by the fact that brefeldin and monensin rapidly increase while cycloheximide tend to decrease the cellular TSP-1 content. T cell TSP-1 is preferentially stored intracellularly and shows variable cell surface expression. T lymphocyte adhesion to fibronectin and collagen type IV induces TSP-1 expression on the cell surface via a brefeldin sensitive mechanism. A monoclonal antibody to TSP-1 inhibits the flattening and pseudopodia formation of the adherent T cells. Furthermore, the same antibody to TSP-1 also exerts an inhibitory effect on T cell migration in the absence of exogenous TSP-1. These results indicate that endogenous TSP-1 is part of an adhesion-dependent mechanism controlling cytoplasmic spreading and migration in T lymphocytes.

Ivanoff, AnnaKarolinska Institutet,Umeå universitet,Klinisk immunologi (författare)
Bergström, Sten-Erik (författare)
Sandström, AndersUmeå universitet,Klinisk immunologi (författare)
Christensson, BirgerKarolinska Institutet (författare)
van Nerven, Joost (författare)
Holgersson, Jan (författare)
Hauzenberger, DanKarolinska Institutet (författare)
Arencibia, IgnacioUmeå universitet,Klinisk immunologi (författare)
Sundqvist, Karl-GöstaKarolinska Institutet,Umeå universitet,Klinisk immunologi (författare)
Umeå universitetKlinisk immunologi (creator_code:org_t)

Av författaren/redakt...: Li, Shu Shun; Ivanoff, Anna; Bergström, Sten- ...; Sandström, Ander ...; Christensson, Bi ...; van Nerven, Joos ...; visa fler...; Holgersson, Jan; Hauzenberger, Da ...; Arencibia, Ignac ...; Sundqvist, Karl- ...; visa färre...

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