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Proteome analysis o...
Proteome analysis of an attenuated Francisella tularensis dsbA mutant : identification of potential DsbA substrate proteins
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- Straskova, Adela (författare)
- Center of Advanced Studies and Institute of Molecular Pathology, Faculty of Military Health Science UO, Czech Republic
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- Pavkova, Ivona (författare)
- Center of Advanced Studies and Institute of Molecular Pathology, Faculty of Military Health Science UO, Czech Republic
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- Link, Marek (författare)
- Center of Advanced Studies and Institute of Molecular Pathology, Faculty of Military Health Science UO, Czech Republic
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- Forslund, Anna-Lena, 1964- (författare)
- Swedish Defence Research Agency, Division of NBC-Defence, 901 82 Umea, Sweden
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- Kuoppa, Kerstin (författare)
- CBRN Defence and Security, FOI Swedish Defence Research Agency, Sweden
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- Noppa, Laila (författare)
- CBRN Defence and Security, FOI Swedish Defence Research Agency, Sweden
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- Kroca, Michal (författare)
- Center of Advanced Studies and Institute of Molecular Pathology, Faculty of Military Health Science UO, Czech Republic
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- Fucikova, Alena (författare)
- Center of Advanced Studies and Institute of Molecular Pathology, Faculty of Military Health Science UO, Czech Republic
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- Klimentova, Jana (författare)
- Center of Advanced Studies and Institute of Molecular Pathology, Faculty of Military Health Science UO, Czech Republic
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- Krocova, Zuzana (författare)
- Center of Advanced Studies and Institute of Molecular Pathology, Faculty of Military Health Science UO, Czech Republic
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- Forsberg, Åke (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Swedish Defence Research Agency, Division of NBC-Defence, 901 82 Umea, Sweden
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- Stulik, Jiri (författare)
- Center of Advanced Studies and Institute of Molecular Pathology, Faculty of Military Health Science UO, Czech Republic
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(creator_code:org_t)
- 2009-10-19
- 2009
- Engelska.
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 8:11, s. 5336-5346
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Francisella tularensis (F. tularensis) is highly infectious for humans via aerosol route and untreated infections with the highly virulent subsp. tularensis can be fatal. Our knowledge regarding key virulence determinants has increased recently but is still somewhat limited. Surface proteins are potential virulence factors and therapeutic targets, and in this study, we decided to target three genes encoding putative membrane lipoproteins in F. tularensis LVS. One of the genes encoded a protein with high homology to the protein family of disulfide oxidoreductases DsbA. The two other genes encoded proteins with homology to the VacJ, a virulence determinant of Shigella flexneri. The gene encoding the DsbA homologue was verified to be required for survival and replication in macrophages and importantly also for in vivo virulence in the mouse infection model for tularemia. Using a combination of classical and shotgun proteome analyses, we were able to identify several proteins that accumulated in fractions enriched for membrane-associated proteins in the dsbA mutant. These proteins are substrate candidates for the DsbA disulfide oxidoreductase as well as being responsible for the virulence attenuation of the dsbA mutant.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Francisella tularensis
- virulence factor
- DsbA
- VacJ
- lipoprotein
- oxidoreductase activity
- 2-DE
- shotgun proteomics
- Molecular biology
- Molekylärbiologi
- molekylärbiologi
- Molecular Biology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Straskova, Adela
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Pavkova, Ivona
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Link, Marek
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Forslund, Anna-L ...
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Kuoppa, Kerstin
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Noppa, Laila
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visa fler...
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Kroca, Michal
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Fucikova, Alena
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Klimentova, Jana
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Krocova, Zuzana
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Forsberg, Åke
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Stulik, Jiri
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Umeå universitet