Sökning: WFRF:(Åhlund Monika K.) >
Directed screen of ...
Directed screen of Francisella novicida virulence determinants using Drosophila melanogaster
-
- Åhlund, Monika K (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
-
- Rydén, Patrik (författare)
- Umeå universitet,Institutionen för matematik och matematisk statistik,Computational Life Science Cluster (CLiC)
-
- Sjöstedt, Anders, 1957- (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
-
visa fler...
-
- Stöven, Svenja, 1964- (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
-
visa färre...
-
(creator_code:org_t)
- ASM International, 2010
- 2010
- Engelska.
-
Ingår i: Infection and Immunity. - : ASM International. - 0019-9567 .- 1098-5522. ; 78:7, s. 3118-3128
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Francisella tularensis is a highly virulent, facultative intracellular human pathogen whose virulence mechanisms are not well understood. Occasional outbreaks of tularemia and the potential use of F. tularensis as a bioterrorist agent warrant better knowledge about the pathogenicity of this bacterium. Thus far, genome-wide in vivo screens for virulence factors have been performed in mice, all however restricted by the necessity to apply competition-based, negative-selection assays. We wanted to individually evaluate putative virulence determinants suggested by such assays and performed directed screening of 249 F. novicida transposon insertion mutants by using survival of infected fruit flies as a measure of bacterial virulence. Some 20% of the genes tested were required for normal virulence in flies; most of these had not previously been investigated in detail in vitro or in vivo. We further characterized their involvement in bacterial proliferation and pathogenicity in flies and in mouse macrophages. Hierarchical cluster analysis of mutant phenotypes indicated a functional linkage between clustered genes. One cluster grouped all but four genes of the Francisella pathogenicity island and other loci required for intracellular survival. We also identified genes involved in adaptation to oxidative stress and genes which might induce host energy wasting. Several genes related to type IV pilus formation demonstrated hypervirulent mutant phenotypes. Collectively, the data demonstrate that the bacteria in part use similar virulence mechanisms in mammals as in Drosophila melanogaster but that a considerable proportion of the virulence factors active in mammals are dispensable for pathogenicity in the insect model.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- tularensis subsp tularensis
- disulfide bond formation
- VI secretion
- in vivo
- schu S4
- pathogenicity island
- Escherichia coli
- identification
- mice
- pathogenesis
- Medical microbiology
- Medicinsk mikrobiologi
- mikrobiologi
- Microbiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas