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Cell specific chemo...
Cell specific chemotyping and multivariate imaging by combined FT-IR microspectroscopy and OPLS analysis reveals the chemical landscape of secondary xylem
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- Gorzsás, András (författare)
- Swedish University of Agricultural Sciences (SLU), Sweden,Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology
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- Stenlund, Hans (författare)
- Umeå universitet,Kemiska institutionen,Computational Life Science Cluster (CLiC)
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- Persson, Per (författare)
- Umeå universitet,Kemiska institutionen
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- Trygg, Johan (författare)
- Umeå universitet,Kemiska institutionen,Computational Life Science Cluster (CLiC)
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- Sundberg, Björn (författare)
- Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU), Sweden
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(creator_code:org_t)
- Blackwell Publishing Ltd, 2011
- 2011
- Engelska.
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Ingår i: The Plant Journal. - : Blackwell Publishing Ltd. - 0960-7412 .- 1365-313X. ; 66:5, s. 903-914
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Fourier-transform infrared (FT-IR) spectroscopy combined with microscopy enables acquiring chemical information from native plant cell walls with high spatial resolution. Combined with a 64 x 64 focal plane array (FPA) detector 4096 spectra from a 0.3 x 0.3 mm image can be simultaneously obtained, where each spectrum represents a compositional and structural "fingerprint" of all cell wall components. For optimal use and analysis of such large amount of information, multivariate approaches are preferred. Here, FT-IR microspectroscopy with FPA detection is combined with orthogonal projections to latent structures discriminant analysis (OPLS-DA). This allows for 1) the extraction of spectra from specific cell types, 2) identification and characterization of different chemotypes using the full spectral information, and 3) further visualising the pattern of identified chemotypes by multivariate imaging. As proof of concept, the chemotypes of Populus tremula xylem cell types are described. The approach revealed unknown features about chemical plasticity and patterns of lignin composition in wood fibers that would have remained hidden in the dataset with traditional data analysis. The applicability of the method on Arabidopsis xylem, and its usefulness in mutant chemotyping is also demonstrated. The methodological approach is not limited to xylem tissues but can be applied to any plant organ/tissue also using other microspectroscopy techniques such as Raman- and UV-microspectroscopy.
Nyckelord
- FT-IR microspectroscopy
- cell wall
- lignin composition
- wood
- poplar
- Arabidopsis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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