SwePub
Sök i LIBRIS databas

  Extended search

id:"swepub:oai:DiVA.org:umu-41642"
 

Search: id:"swepub:oai:DiVA.org:umu-41642" > Dominant expression...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Hosokawa, KUmeå universitet,Obstetrik och gynekologi (author)

Dominant expression and distribution of oestrogen receptor beta over oestrogen receptor alpha in the human corpus luteum

  • Article/chapterEnglish2001

Publisher, publication year, extent ...

  • Oxford University Press (OUP),2001
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:umu-41642
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-41642URI
  • https://doi.org/10.1093/molehr/7.2.137DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • To investigate the potential importance of oestrogen as a local regulator of human corpus luteum function, the mRNA expression pattern and cellular localization of oestrogen receptors (ERs), ER-alpha and ER-beta, were studied in corpora lutea grouped according to age, where days 2-5 post-LH rise were designated as the early luteal phase, days 6-10 as mid-luteal and days 11-14 as the late luteal phase respectively. Northern blot analysis using an ER-beta probe in samples from whole ovarian tissue and isolated corpora lutea, revealed a major band at 7.5 kb and several minor bands between 4-10 kb, while no signals for ER-alpha mRNA were obtained. However, using a semi-quantitative reverse transcription-polymerase chain reaction followed by Southern blotting, ER-beta mRNA levels were found to be 63% lower (P: < 0.05, n = 39) in the mid-luteal phase compared with the early luteal phase, while ER-alpha mRNA expression showed no statistical differences between the different age groups. Using in-situ hybridization, ER-beta mRNA expression was localized to the steroidogenic luteal cells as well as perivascular cells and fibroblasts in the corpus luteum. Immunohistochemistry confirmed the localization of ER-beta protein, but no clear staining of luteal cells was found using antibodies against ER-alpha. Collectively, the findings of low to moderate expression of ER-beta mRNA and protein in the steroidogenic cells, and also in vascular endothelial cells of the corpus luteum, as opposed to diminutive amounts of ER-alpha mRNA, suggest that oestrogen activity is primarily transduced via ER-beta in the human corpus luteum.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Ottander, UlrikaUmeå universitet,Obstetrik och gynekologi(Swepub:umu)ulaotr95 (author)
  • Wahlberg, PUmeå universitet,Institutionen för medicinsk biovetenskap (author)
  • Ny, TorUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)tony0001 (author)
  • Cajander, SUmeå universitet,Patologi (author)
  • Olofsson, Jan IUmeå universitet,Obstetrik och gynekologi (author)
  • Umeå universitetObstetrik och gynekologi (creator_code:org_t)

Related titles

  • In:Molecular human reproduction: Oxford University Press (OUP)7:2, s. 137-1451360-99471460-2407

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Find more in SwePub

By the author/editor
Hosokawa, K
Ottander, Ulrika
Wahlberg, P
Ny, Tor
Cajander, S
Olofsson, Jan I
About the subject
MEDICAL AND HEALTH SCIENCES
MEDICAL AND HEAL ...
and Clinical Medicin ...
and Obstetrics Gynae ...
Articles in the publication
Molecular human ...
By the university
Umeå University

Search outside SwePub

Wikipedia about the author:
K_Hosokawa
Hosokawa, K
en

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view