Sökning: WFRF:(Antti Henrik 1970 )
> (2010-2014) >
Pair-wise multicomp...
-
Shiryaeva, Liudmila,1970-Umeå universitet,Kemiska institutionen
(författare)
Pair-wise multicomparison and OPLS analyses of cold-acclimation phases in Siberian spruce
- Artikel/kapitelEngelska2012
Förlag, utgivningsår, omfång ...
-
2011-04-11
-
Springer,2012
-
electronicrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:umu-43509
-
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-43509URI
-
https://doi.org/10.1007/s11306-011-0304-5DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Published online 11 april 2011. Copywright, The Author(s) 2011. This article is published with open access at Springerlink.com
-
Analysis of metabolomics data often goes beyond the task of discovering biomarkers and can be aimed at recovering other important characteristics of observed metabolomic changes. In this paper we explore different methods to detect the presence of distinctive phases in seasonal-responsive changes of metabolomic patterns of Siberian spruce (Picea obovata) during cold acclimation occurred in the period from mid-August to January. Multivariate analysis, specifically orthogonal projection to latent structures discriminant analysis (OPLSDA), identified time points where the metabolomic patterns underwent substantial modifications as a whole, revealing four distinctive phases during acclimation. This conclusion was re-examined by a univariate analysis consisting of multiple pair-wise comparisons to identify homogeneity intervals for each metabolite. These tests complemented OPLS-DA, clarifying biological interpretation of the classification: about 60% of metabolites found responsive to the cold stress indeed changed at one or more of the time points predicted by OPLS-DA. However, the univariate approach did not support the proposed division of the acclimation period into four phases: less than 10% of metabolites altered during the acclimation had homogeneous levels predicted by OPLS-DA. This demonstrates that coupling the classification found by OPLS-DA and the analysis of dynamics of individual metabolites obtained by pair-wise multicomparisons reveals a more correct characterization of biochemical processes in freezing tolerant trees and leads to interpretations that cannot be deduced by either method alone. The combined analysis can be used in other ‘omics’-studies, where response factors have a causal dependence (like the time in the present work) and pairwise multicomparisons are not conservative.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Antti, Henrik,1970-Umeå universitet,Kemiska institutionen(Swepub:umu)hean0004
(författare)
-
Schröder, Wolfgang PUmeå universitet,Kemiska institutionen(Swepub:umu)wosc0001
(författare)
-
Strimbeck, G RichardDepartment of Biology, Norwegian University of Science and Technology
(författare)
-
Shiryaev, Anton SDepartment of Engineering Cybernetics, Norwegian University of Science and Technology
(författare)
-
Umeå universitetKemiska institutionen
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Metabolomics: Springer8:Suppl 1, s. 123-1301573-38821573-3890
Internetlänk
Hitta via bibliotek
Till lärosätets databas