Search: onr:"swepub:oai:DiVA.org:umu-46224" >
An assessment of hi...
-
Egelhofer, Thea A
(author)
An assessment of histone-modification antibody quality
- Article/chapterEnglish2011
Publisher, publication year, extent ...
-
2010-12-05
-
Springer Science and Business Media LLC,2011
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:umu-46224
-
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-46224URI
-
https://doi.org/10.1038/nsmb.1972DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).
Added entries (persons, corporate bodies, meetings, titles ...)
-
Minoda, Aki
(author)
-
Klugman, Sarit
(author)
-
Lee, Kyungjoon
(author)
-
Kolasinska-Zwierz, Paulina
(author)
-
Alekseyenko, Artyom A
(author)
-
Cheung, Ming-Sin
(author)
-
Day, Daniel S
(author)
-
Gadel, Sarah
(author)
-
Gorchakov, Andrey A
(author)
-
Gu, Tingting
(author)
-
Kharchenko, Peter V
(author)
-
Kuan, Samantha
(author)
-
Latorre, Isabel
(author)
-
Linder-Basso, Daniela
(author)
-
Luu, Ying
(author)
-
Ngo, Queminh
(author)
-
Perry, Marc
(author)
-
Rechtsteiner, Andreas
(author)
-
Riddle, Nicole C
(author)
-
Schwartz, Yuri BRutgers University, New Jersey, U.S.A.(Swepub:umu)yusc0002
(author)
-
Shanower, Gregory A
(author)
-
Vielle, Anne
(author)
-
Ahringer, Julie
(author)
-
Elgin, Sarah C R
(author)
-
Kuroda, Mitzi I
(author)
-
Pirrotta, Vincenzo
(author)
-
Ren, Bing
(author)
-
Strome, Susan
(author)
-
Park, Peter J
(author)
-
Karpen, Gary H
(author)
-
Hawkins, R David
(author)
-
Lieb, Jason D
(author)
-
Rutgers University, New Jersey, U.S.A.
(creator_code:org_t)
Related titles
-
In:Nature Structural & Molecular Biology: Springer Science and Business Media LLC18:1, s. 91-931545-99931545-9985
Internet link
Find in a library
To the university's database
- By the author/editor
-
Egelhofer, Thea ...
-
Minoda, Aki
-
Klugman, Sarit
-
Lee, Kyungjoon
-
Kolasinska-Zwier ...
-
Alekseyenko, Art ...
-
show more...
-
Cheung, Ming-Sin
-
Day, Daniel S
-
Gadel, Sarah
-
Gorchakov, Andre ...
-
Gu, Tingting
-
Kharchenko, Pete ...
-
Kuan, Samantha
-
Latorre, Isabel
-
Linder-Basso, Da ...
-
Luu, Ying
-
Ngo, Queminh
-
Perry, Marc
-
Rechtsteiner, An ...
-
Riddle, Nicole C
-
Schwartz, Yuri B
-
Shanower, Gregor ...
-
Vielle, Anne
-
Ahringer, Julie
-
Elgin, Sarah C R
-
Kuroda, Mitzi I
-
Pirrotta, Vincen ...
-
Ren, Bing
-
Strome, Susan
-
Park, Peter J
-
Karpen, Gary H
-
Hawkins, R David
-
Lieb, Jason D
-
show less...
- Articles in the publication
-
Nature Structura ...
- By the university
-
Umeå University