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- Bunikis, IgnasUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström (author)
Multiplex PCR as a tool for validating plasmid content of Borrelia burgdorferi.
- Article/chapterEnglish2011
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- Elsevier BV,2011
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- LIBRIS-ID:oai:DiVA.org:umu-46238
- https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-46238URI
- https://doi.org/10.1016/j.mimet.2011.05.004DOI
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- Language:English
- Summary in:English
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- Borrelia burgdorferi has an unusual genomic structure containing 21 plasmids. These plasmids carry genes that are essential for infectivity and survival of the spirochetes in vivo. Several plasmids are lost during cultivation in vitro, which might lead to a heterogeneous population after multiple passages and loss of infectivity in laboratory animals. Herein, we present a simple and inexpensive multiplex PCR method that detects the complete plasmid profile of B. burgdorferi B31 in just two PCR tubes.
Added entries (persons, corporate bodies, meetings, titles ...)
- Bonde, MariUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström(Swepub:umu)maiboe01 (author)
- Kutschan-Bunikis, SabrinaUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström(Swepub:umu)saku0021 (author)
- Bergström, SvenUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström(Swepub:umu)svbe0001 (author)
- Umeå universitetInstitutionen för molekylärbiologi (Medicinska fakulteten) (creator_code:org_t)
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- In:Journal of Microbiological Methods: Elsevier BV86:2, s. 243-70167-70121872-8359
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