Search: L773:0167 7012 OR L773:1872 8359
> (2010-2014) >
Multiplex PCR as a ...
Multiplex PCR as a tool for validating plasmid content of Borrelia burgdorferi.
-
- Bunikis, Ignas (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström
-
- Bonde, Mari (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström
-
- Kutschan-Bunikis, Sabrina (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström
-
show more...
-
- Bergström, Sven (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2011
- 2011
- English.
-
In: Journal of Microbiological Methods. - : Elsevier BV. - 0167-7012 .- 1872-8359. ; 86:2, s. 243-7
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Borrelia burgdorferi has an unusual genomic structure containing 21 plasmids. These plasmids carry genes that are essential for infectivity and survival of the spirochetes in vivo. Several plasmids are lost during cultivation in vitro, which might lead to a heterogeneous population after multiple passages and loss of infectivity in laboratory animals. Herein, we present a simple and inexpensive multiplex PCR method that detects the complete plasmid profile of B. burgdorferi B31 in just two PCR tubes.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database