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Attachment of HeT-A...
Attachment of HeT-A sequences to chromosomal termini in Drosophila melanogaster may occur by different mechanisms
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- Kahn, Tatiana (författare)
- Institute of Gene Biology, RAS, Russia
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- Savitsky, Mikhail (författare)
- Institute of Gene Biology, RAS, Russia
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- Georgiev, Pavel (författare)
- Institute of Gene Biology, RAS, Russia
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(creator_code:org_t)
- 2000
- 2000
- Engelska.
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Ingår i: Molecular and Cellular Biology. - 0270-7306 .- 1098-5549. ; 20:20, s. 7634-7642
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Drosophila telomeres contain arrays of the retrotransposonlike elements HeT-A and TART. Their transposition to broken chromosomal termini has been implicated in chromosome healing and telomere elongation. The HeT-A element is attached by its 3' end, which contains the promoter. To monitor the behavior of HeT-A elements, we used the yellow gene with terminal deficiencies consisting of breaks in the yellow promoter region that result in the y-null phenotype. Attachment of the HeT-A element provides the promoterless yellow gene with a promoter that activates yellow expression in bristles. The frequency of HeT-A transpositions to the yellow terminal deficiency depends on the genotype of the line and varies from 2 x 10(-3) to less than 2 x 10(-5). Loss of the attached HeT-A due to incomplete replication at the telomere leads to inactivation of yellow expression, which is restored by attachment of a new HeT-A element upstream of yellow. New HeT-A additions occur at a frequency of about 1.2 x 10(-3). Short DNA attachments are generated by gene conversion using the homologous telomeric sequences as templates. Longer DNA attachments are generated either by conventional transposition of an HeT-A element to the chromosomal terminus or by recombination between the 3' terminus of telomeric HeT-A elements and the receding end of HeT-A attached to the yellow gene.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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- art (ämneskategori)
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