Analyzing P-bodies in Saccharomyces cerevisiae

Nissan, TracyThe University of Arizona, Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, Tucson, Arizona, USA (författare)

Cytoplasmic processing bodies, or P-bodies, are RNA-protein granules found in eukaryotic cells. P-bodies contain non-translating mRNAs and proteins involved in mRNA degradation and translational repression. P-bodies, and the mRNPs within them, have been implicated in mRNA storage, mRNA degradation, and translational repression. The analysis of mRNA turnover often involves the analysis of P-bodies. In this chapter, we describe methods to analyze P-bodies in the budding yeast, Saccharomyces cerevisiae, including procedures to determine whether a protein or mRNA can accumulate in P-bodies, whether an environmental perturbation or mutation affects P-body size and number, and methods to quantify P-bodies.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
cytoplasmic processing bodies messenger-rna decay translational repression human-cells gene-expression global analysis body formation budding yeast localization stability

Parker, Roy (författare)
The University of Arizona, Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, Tucson, Arizona, USA (creator_code:org_t)

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